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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/1055

Title: A note on challenge trials to determine the growth of Listeria monocytogenes on mushrooms (Agaricus bisporus)
Authors: Leong, Dara
Alvarez-Ordonez, Avelino
Jordan, Kieran
Keywords: Listeria monocytogenes
Agaricus bisporus
challenge study
Food safety
Issue Date: 30-Dec-2015
Publisher: Teagasc (Agriculture and Food Development Authority), Ireland
Citation: Leong, D., Alvarez-Ordóñez, A. & Jordan, K. (2015). A note on challenge trials to determine the growth of Listeria monocytogenes on mushrooms (Agaricus bisporus). Irish Journal of Agricultural and Food Research, 54(2), pp. 121-125. doi:10.1515/ijafr-2015-0013
Series/Report no.: Irish Journal of Agricultural and Food Research;vol 54
Abstract: In the EU, food is considered safe with regard to Listeria monocytogenes if the number of micro-organisms does not exceed 100 colony forming units (cfu)/g throughout its shelf-life. Therefore, it is important to determine if a food supports growth of L. monocytogenes. Guidelines for conducting challenge tests for growth assessment of L. monocytogenes on foods were published by the European Union Reference Laboratory (EURL) in 2014. The aim of this study was to use these guidelines to determine if refrigerated, fresh, whole, closed-cap, prepackaged mushrooms (Agaricus bisporus) support the growth of L. monocytogenes. Three batches of mushrooms were artificially inoculated at approximately 100 cfu/g with a three-strain mix of L. monocytogenes and incubated for 2 days at 8°C followed by 4 days at 12°C. L. monocytogenes numbers were determined (in triplicate for each batch) on days 0, 2 and 6. Water activity, pH and total bacterial counts were also determined. There was no increase in the number of L. monocytogenes above the threshold of 0.5 log cfu/g in any of the replicates. In 8 of 9 replicates, the numbers decreased indicating that A. bisporus do not support the growth of L. monocytogenes. As the EU regulations allow < 100 cfu/g if the food cannot support growth of L. monocytogenes, the significance of this study is that mushrooms with < 100 cfu/g may be within the regulations and therefore, quantitative rather than qualitative determination may be required.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/1055
ISSN: 2009-9029
Appears in Collections:IJAFR, volume 54, no 2, 2015
Food Quality & Sensory Science

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