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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/1102

Title: Genetic basis of benzimidazole resistance in Teladorsagia circumcincta in Ireland
Authors: Keegan, Jason D
Good, Barbara
de Waal, Theo
Fanning, June
Keane, Orla M
Keywords: Sheep
Anthelmintic
Resistance
Benzimidazole
Teladorsagia
β-tubulin
Issue Date: 13-Feb-2017
Publisher: Biomed Central
Citation: Keegan, Jason D. et al.Genetic basis of benzimidazole resistance in Teladorsagia circumcincta in Ireland. Irish Veterinary Journal. 2017 Feb 13;70(1):8. DOI: 10.1186/s13620-017-0087-8
Series/Report no.: Irish Veterinary Journal;vol 70
Abstract: Resistance to benzimidazole (BZ) anthelmintics is common in ovine nematodes of economic importance. Single nucleotide polymorphisms (SNP) at three positions in the isotype 1 β– tubulin gene have been associated with BZ resistance and molecular tests for the detection of BZ resistance have been developed. In order to determine if such tests are practicable in Ireland the polymorphisms associated with BZ resistance must be identified. To this end, BZ-resistant nematodes were recovered from four farms in Ireland. Resistant Teladorsagia circumcincta, Cooperia curticei and Trichostrongylus colubriformis were recovered, with resistant T. circumcincta the most common and the only species studied further. Sequencing of the isotype 1 β–tubulin gene from resistant T. circumcincta identified a T - A transition, resulting in an F200Y substitution known to be responsible for BZ-resistance, on three of the farms. However, on the fourth farm the frequency of the resistant A allele was only 0.33 indicating another BZ resistance mechanism may be present on this farm. An additional polymorphism resulting in a substitution of glutamate for leucine (E198L) was also found on this farm at low frequency (0.17). No polymorphisms at position 167 were identified on any farm. Therefore, molecular tests to detect BZ resistance in T. circumcincta in Ireland could prove useful; however, they may result in some instances of resistance remaining undetected.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/1102
http://dx.doi.org/10.1186/s13620-017-0087-8
Appears in Collections:Animal & Bioscience
Teagasc publications in Biomed Central

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