Food Programme >
Food Safety >
Please use this identifier to cite or link to this item:
|Title: ||Complete Genome Sequence of Clostridium estertheticum DSM 8809, a Microbe Identified in Spoiled Vacuum Packed Beef|
|Authors: ||Zhongyi, Yu|
Wall, Patrick G.
|Keywords: ||blown pack spoilage|
vacuum packed beef
whole genome sequencing
|Issue Date: ||10-Nov-2016|
|Citation: ||Yu Zhongyi, Gunn Lynda, Brennan Evan, Reid Rachael, Wall Patrick G., Gaora Peadar Ó., Hurley Daniel, Bolton Declan, Fanning Séamus. Complete Genome Sequence of Clostridium estertheticum DSM 8809, a Microbe Identified in Spoiled Vacuum Packed Beef . Frontiers in Microbiology , 2016, 7, 1764. DOI=10.3389/fmicb.2016.01764|
|Series/Report no.: ||Frontiers in Microbiology;vol 7|
|Abstract: ||Blown pack spoilage (BPS) is a major issue for the beef industry. Etiological agents
of BPS involve members of a group of Clostridium species, including Clostridium
estertheticum which has the ability to produce gas, mostly carbon dioxide, under
anaerobic psychotrophic growth conditions. This spore-forming bacterium grows slowly
under laboratory conditions, and it can take up to 3 months to produce a workable
culture. These characteristics have limited the study of this commercially challenging
bacterium. Consequently information on this bacterium is limited and no effective
controls are currently available to confidently detect and manage this production risk.
In this study the complete genome of C. estertheticum DSM 8809 was determined
R sequencing. The genome consists of a circular chromosome of 4.7 Mbp
along with a single plasmid carrying a potential tellurite resistance gene tehB and a Tn3-
like resolvase-encoding gene tnpR. The genome sequence was searched for central
metabolic pathways that would support its biochemical profile and several enzymes
contributing to this phenotype were identified. Several putative antibiotic/biocide/metal
resistance-encoding genes and virulence factors were also identified in the genome,
a feature that requires further research. The availability of the genome sequence will
provide a basic blueprint from which to develop valuable biomarkers that could support
and improve the detection and control of this bacterium along the beef production chain.|
The funding for this research was provided by the Food Institutional Research Measure (FIRM) administered by the Department of Agriculture, Food and the Marine (Ireland).
|Appears in Collections:||Food Safety|
Items in T-Stor are protected by copyright, with all rights reserved, unless otherwise indicated.