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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/1251

Title: Whey protein effects on energy balance link the intestinal mechanisms of energy absorption with adiposity and hypothalamic neuropeptide gene expression
Authors: Nilaweera, Kanishka N
Cabrera-Rubio, Raul
Speakman, John R.
O'Connor, Paula M.
McAuliffe, Ann Marie
Guinane, Catriona M.
Lawton, Elaine M.
Crispie, Fiona
Aguilera, Monica
Stanley, Maurice
Boscaini, Serena
Joyce, Susan
Melgar, Sylvia
Cryan, John F.
Cotter, Paul D.
Keywords: Whey proteins
energy balance
intestine
gut microbiota
gene expression
Issue Date: 21-Mar-2017
Publisher: American Physiological Society
Citation: Nilaweera KN, Cabrera-Rubio R, Speakman JR, O' Connor PM, McAuliffe A, Guinane CM, Lawton E, Crispie F, Aguilera M, Stanley M and others. Whey protein-effects on energy balance link the intestinal mechanisms of energy absorption with adiposity and hypothalamic neuropeptide gene expression. American journal of Physiology. Endocrinology and Metabolism 2017;313(1):E1-E11; doi 10.1152/ajpendo.00356.2016.
Series/Report no.: American Journal of Physiology - Endocrinology and Metabolism;vol 31
Abstract: We tested the hypothesis that dietary whey protein isolate (WPI) affects the intestinal mechanisms related to energy absorption and that the resulting energy deficit is compensated by changes in energy balance to support growth. C57BL/6 mice were provided a diet enriched with WPI with varied sucrose content, and the impact on energy balance-related parameters was investigated. As part of a high-sucrose diet, WPI reduced the hypothalamic expression of pro-opiomelanocortin gene expression and increased energy intake. The energy expenditure was unaffected, but epididymal weight was reduced, indicating an energy loss. Notably, there was a reduction in the ileum gene expression for amino acid transporter SLC6a19, glucose transporter 2, and fatty acid transporter 4. The composition of the gut microbiota also changed, where Firmicutes were reduced. The above changes indicated reduced energy absorption through the intestine. We propose that this mobilized energy in the adipose tissue and caused hypothalamic changes that increased energy intake, acting to counteract the energy deficit arising in the intestine. Lowering the sucrose content in the WPI diet increased energy expenditure. This further reduced epididymal weight and plasma leptin, whereupon hypothalamic ghrelin gene expression and the intestinal weight were both increased. These data suggest that when the intestine-adipose-hypothalamic pathway is subjected to an additional energy loss (now in the adipose tissue), compensatory changes attempt to assimilate more energy. Notably, WPI and sucrose content interact to enable the component mechanisms of this pathway.
Description: peer-reviewed
This work was supported by Teagasc, Ireland and in part by a research grant from Science Foundation Ireland (SFI) under the Grant Number SFI/16/BBSRC/3389 and the BBSRC under the Grant Reference BB/P009875/1 (KNN and JRS) and by SFI Grant Number SFI/12/RC/2273 (JFC and APC Microbiome Institute).
URI: http://hdl.handle.net/11019/1251
http://dx.doi.org/10.1152/ajpendo.00356.2016
Appears in Collections:Food Biosciences

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