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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/1516

Title: Illumina MiSeq Phylogenetic Amplicon Sequencing Shows a Large Reduction of an Uncharacterised Succinivibrionaceae and an Increase of the Methanobrevibacter gottschalkii Clade in Feed Restricted Cattle
Authors: McCabe, Matthew Sean
Cormican, Paul
Keogh, Kate
O'Connor, Aaron
O'Hara, Eoin
Palladino, Rafael Alejandro
Kenny, David A.
Waters, Sinead M.
Keywords: Periodic feed restriction
Cattle
compensatory growth
Illumina MiSeq Phylogenetic Amplicon Sequencing
Methanobrevibacter gottschalkii clade
uncharacterised Succinivibrionaceae species
acetate:propionate ratio
feed efficiency
Issue Date: 30-Jul-2015
Publisher: PLOS
Citation: McCabe MS, Cormican P, Keogh K, O’Connor A, O’Hara E, Palladino RA, et al. (2015) Illumina MiSeq Phylogenetic Amplicon Sequencing Shows a Large Reduction of an Uncharacterised Succinivibrionaceae and an Increase of the Methanobrevibacter gottschalkii Clade in Feed Restricted Cattle. PLoS ONE 10(7): e0133234. https://doi.org/10.1371/journal.pone.0133234
Series/Report no.: PLoS ONE;vol 10
Abstract: Periodic feed restriction is used in cattle production to reduce feed costs. When normal feed levels are resumed, cattle catch up to a normal weight by an acceleration of normal growth rate, known as compensatory growth, which is not yet fully understood. Illumina Miseq Phylogenetic marker amplicon sequencing of DNA extracted from rumen contents of 55 bulls showed that restriction of feed (70% concentrate, 30% grass silage) for 125 days, to levels that caused a 60% reduction of growth rate, resulted in a large increase of relative abundance of Methanobrevibacter gottschalkii clade (designated as OTU-M7), and a large reduction of an uncharacterised Succinivibrionaceae species (designated as OTU-S3004). There was a strong negative Spearman correlation (ρ = -0.72, P = <1x10-20) between relative abundances of OTU-3004 and OTU-M7 in the liquid rumen fraction. There was also a significant increase in acetate:propionate ratio (A:P) in feed restricted animals that showed a negative Spearman correlation (ρ = -0.69, P = <1x10-20) with the relative abundance of OTU-S3004 in the rumen liquid fraction but not the solid fraction, and a strong positive Spearman correlation with OTU-M7 in the rumen liquid (ρ = 0.74, P = <1x10-20) and solid (ρ = 0.69, P = <1x10-20) fractions. Reduced A:P ratios in the rumen are associated with increased feed efficiency and reduced production of methane which has a global warming potential (GWP 100 years) of 28. Succinivibrionaceae growth in the rumen was previously suggested to reduce methane emissions as some members of this family utilise hydrogen, which is also utilised by methanogens for methanogenesis, to generate succinate which is converted to propionate. Relative abundance of OTU-S3004 showed a positive Spearman correlation with propionate (ρ = 0.41, P = <0.01) but not acetate in the liquid rumen fraction.
Description: peer-reviewed
This study was supported by the Science Foundation Ireland (http://www.sfi.ie) (Contract number SFI 09/RFP/GEN2447-awarded to SMW) and Teagasc Walsh Fellowship Funding (www.teagasc.ie) (Teagasc project RMIS 6341-awarded to SMW).
URI: http://hdl.handle.net/11019/1516
https://doi.org/10.1371/journal.pone.0133234
Appears in Collections:Animal & Bioscience

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