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dc.contributor.authorMatthews, Daragh
dc.contributor.authorDiskin, Michael G
dc.contributor.authorKenny, David A
dc.contributor.authorCreevey, Christopher J
dc.contributor.authorKeogh, Kate
dc.contributor.authorWaters, Sinead M
dc.date.accessioned2018-01-11T09:55:14Z
dc.date.available2018-01-11T09:55:14Z
dc.date.issued2017-11-09
dc.identifier.citationMatthews D, Diskin MG, Kenny DA, Creevey CJ, Keogh K, Waters SM. Effect of short term diet restriction on gene expression in the bovine hypothalamus using next generation RNA sequencing technology. Bmc Genomics 2017;18; doi 10.1186/s12864-017-4265-6.en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1527
dc.identifier.urihttp://dx.doi.org/10.1186/s12864-017-4265-6
dc.descriptionpeer-revieweden_GB
dc.descriptionThis work was funded through Teagasc Walsh Fellowship to Daragh Matthews (Project Number: RMIS 5756).
dc.description.abstractBackground Negative energy balance (NEB) is an imbalance between energy intake and energy requirements for lactation and body maintenance affecting high-yielding dairy cows and is of considerable economic importance due to its negative impact on fertility and health in dairy herds. It is anticipated that the cow hypothalamus experiences extensive biochemical changes during the early post partum period in an effort to re-establish metabolic homeostasis. However, there is variation in the tolerance to NEB between individual cows. In order to understand the genomic regulation of ovulation in hypothalamic tissue during NEB, mRNA transcriptional patterns between tolerant and sensitive animals were examined. A short term dietary restriction heifer model was developed which induced abrupt onset of anoestrus in some animals (Restricted Anovulatory; RA) while others maintained oestrous cyclicity (Restricted Ovulatory; RO). A third control group (C) received a higher level of normal feeding. Results A total of 15,295 genes were expressed in hypothalamic tissue. Between RA and C groups 137 genes were differentially expressed, whereas between RO and C, 32 genes were differentially expressed. Differentially expressed genes were involved in the immune response and cellular motility in RA and RO groups, respectively, compared to C group. The largest difference between groups was observed in the comparison between RA and RO heifers, with 1094 genes shown to be significantly differentially expressed (SDE). Pathway analysis showed that these SDE genes were associated with 6 canonical pathways (P < 0.01), of which neuroactive ligand-receptor interaction was the most significant. Within the comparisons the main over-represented pathway functions were immune response including neuroprotection (CXCL10, Q1KLR3, IFIH1, IL1 and IL8; RA v C and RA v RO); energy homeostasis (AgRP and NPY; RA v RO); cell motility (CADH1, DSP and TSP4; RO v C) and prevention of GnRH release (NTSR1 IL1α, IL1β, NPY and PACA; RA v RO). Conclusions This information will assist in understanding the genomic factors regulating the influence of diet restriction on fertility and may assist in optimising nutritional and management systems for the improvement in reproductive performance.en_GB
dc.description.sponsorshipTeagasc Walsh Fellowship Programmeen_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Genomics;vol 18
dc.subjectmRNAseqen_GB
dc.subjectBovineen_GB
dc.subjectHypothalamusen_GB
dc.subjectEnergy homeostasisen_GB
dc.subjectReproductionen_GB
dc.subjectDietary Restrictionen_GB
dc.titleEffect of short term diet restriction on gene expression in the bovine hypothalamus using next generation RNA sequencing technologyen_GB
dc.typeArticleen_GB
dc.date.updated2017-11-12T05:39:05Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
refterms.dateFOA2018-01-12T08:57:52Z


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