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dc.contributor.authorSurlis, Carla
dc.contributor.authorMcNamara, Keelan
dc.contributor.authorO’Hara, Eoin
dc.contributor.authorWaters, Sinead
dc.contributor.authorBeltman, Marijke
dc.contributor.authorCassidy, Joseph
dc.contributor.authorKenny, David
dc.date.accessioned2018-01-11T09:59:00Z
dc.date.available2018-01-11T09:59:00Z
dc.date.issued2017-12-14
dc.identifier.citationSurlis C, McNamara K, O’Hara E, Waters S, Beltman M, Cassidy J, Kenny D. Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves. BMC Veterinary Research 2017;13(1):391; doi 10.1186/s12917-017-1310-2.en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1528
dc.identifier.urihttp://dx.doi.org/10.1186/s12917-017-1310-2
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Caesarean section is a routine veterinary obstetrical procedure employed to alleviate dystocia in cattle. However, CS, particularly before the onset of labour, is known to negatively affect neonatal respiration and metabolic adaptation in humans, though there is little published information for cattle. The aim of this study was to investigate the effect of elective caesarean section (ECS) or normal trans-vaginal (TV) delivery, on lung and jejunal gene expression profiles of neonatal calves. Results Paternal half-sib Angus calves (gestation length 278 + 1.8 d) were delivered either transvaginally (TV; n = 8) or by elective caesarean section (ECS; n = 9) and immediately euthanized. Lung and jejunum epithelial tissue was isolated and snap frozen. Total RNA was extracted using Trizol reagent and reverse transcribed to generate cDNA. For lung tissue, primers were designed to target genes involved in immunity, surfactant production, cellular detoxification, membrane transport and mucin production. Primers for jejunum tissue were chosen to target mucin production, immunoglobulin uptake, cortisol reaction and membrane trafficking. Quantitative real-time PCR reactions were performed and data were statistically analysed using mixed models ANOVA. In lung tissue the expression of five genes were affected (p < 0.05) by delivery method. Four of these genes were present at lower (LAP, CYP1A1, SCN11α and SCN11β) and one (MUC5AC) at higher abundance in ECS compared with TV calves. In jejunal tissue, expression of TNFα, Il-1β and 1 l-6 was higher in ECS compared with TV calves. Conclusions This novel study shows that ECS delivery affects the expression of key genes involved in the efficiency of the pulmonary liquid to air transition at birth, and may lead to an increased inflammatory response in jejunal tissue, which could compromise colostral immunoglobulin absorption. These findings are important to our understanding of the viability and management of neonatal calves born through ECS.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Veterinary Research;
dc.subjectMode of delivery –en_GB
dc.subjectElective caesareanen_GB
dc.subjectCalfen_GB
dc.subjectBovineen_GB
dc.subjectlungen_GB
dc.subjectJejunumen_GB
dc.subjectBirthen_GB
dc.titleBirth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calvesen_GB
dc.typeArticleen_GB
dc.date.updated2017-12-17T04:54:13Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
refterms.dateFOA2018-01-12T08:57:30Z


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