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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/229

Title: Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection
Authors: Rosberg-Cody, Eva
Liavonchanka, Alena
Gobel, Cornelia
Ross, R Paul
O'Sullivan, Orla
Fitzgerald, Gerald F
Feussner, Ivo
Stanton, Catherine
Keywords: Myosin-cross-reactive antigen
Conjugated linoleic acid
Bacterial stress protection
Issue Date: 17-Feb-2011
Publisher: Biomed Central
Citation: Rosberg-Cody, Eva et al. Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection. BMC Biochemistry, 2011, 12(1):9. DOI:10.1186/1471-2091-12-9
Series/Report no.: BMC Biochemistry
Abstract: Background The aim of this study was to determine the catalytic activity and physiological role of myosin-cross-reactive antigen (MCRA) from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned, sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium) and the recombinant proteins assessed for enzymatic activity against fatty acid substrates. Results MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls. Conclusions MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production, and this protein has an additional function in bacterial stress protection.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/229
ISSN: 1471-2091
Appears in Collections:Teagasc publications in Biomed Central
Food Biosciences

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