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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/248

Title: Long-term stability of RNA in post-mortem bovine skeletal muscle, liver and subcutaneous adipose tissues
Authors: Bahar, Bojlul
Monahan, Frank J
Moloney, Aidan P
Schmidt, Olaf
MacHugh, David E
Sweeney, Torres
Keywords: Post-mortem RNA stability
Bovine Tissue
Issue Date: 29-Nov-2007
Publisher: Biomed Central
Citation: Bojlul Bahar, Frank J Monahan, Aidan P Moloney, Olaf Schmidt, David E MacHugh and Torres Sweeney. Long-term stability of RNA in post-mortem bovine skeletal muscle, liver and subcutaneous adipose tissues. BMC Molecular Biology 2007, 8:108. DOI:10.1186/1471-2199-8-108
Series/Report no.: BMC Molecular Biology
Abstract: Background: Recovering high quality intact RNA from post-mortem tissue is of major concern for gene expression studies in animals and humans. Since the availability of post-mortem tissue is often associated with substantial delay, it is important that we understand the temporal variation in the stability of total RNA and of individual gene transcripts so as to be able to appropriately interpret the data generated from such studies. Hence, the objective of this experiment was to qualitatively and quantitatively assess the integrity of total and messenger RNA extracted from bovine skeletal muscle, subcutaneous adipose tissue and liver stored at 4°C at a range of time points up to 22 days post-mortem. These conditions were designed to mimic the environment prevailing during the transport of beef from the abattoir to retail outlets. Results: The 28S and 18S rRNA molecules of total RNA were intact for up to 24 h post-mortem in liver and adipose tissues and up to 8 days post-mortem in skeletal muscle. The mRNA of housekeeping genes (GAPDH and ACTB) and two diet-related genes (RBP5 and SCD) were detectable up to 22 days post-mortem in skeletal muscle. While the mRNA stability of the two housekeeping genes was different in skeletal muscle and liver, they were similar to each other in adipose tissue. After 22 days post-mortem, the relative abundance of RBP5 gene was increased in skeletal muscle and in adipose tissue and decreased in liver. During this period, the relative abundance of SCD gene also increased in skeletal muscle whereas it decreased in both adipose tissue and liver. Conclusion: Stability of RNA in three tissues (skeletal muscle, subcutaneous adipose tissue and liver) subjected to long-term post-mortem storage at refrigeration temperature indicated that skeletal muscle can be a suitable tissue for recovering biologically useful RNA for gene expression studies even if the tissue is subjected to post-mortem storage for weeks, whereas adipose tissue and liver should be processed within 24 hours post-mortem.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/248
http://dx.doi.org/10.1186/1471-2199-8-108
Appears in Collections:Animal & Bioscience
Teagasc publications in Biomed Central

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