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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/254

Title: A general method for selection of riboflavin-overproducing food grade micro-organisms
Authors: Burgess, Catherine M
Smid, Eddy J
Rutten, Ger
van Sinderen, Douwe
Keywords: Riboflavin
Issue Date: 18-Jul-2006
Publisher: Biomed Central
Citation: Catherine M Burgess, Eddy J Smid, Ger Rutten and Douwe van Sinderen. A general method for selection of riboflavin-overproducing food grade micro-organisms. Microbial Cell Factories, 2006, 5(1):24
Series/Report no.: Microbial Cell Factories
Abstract: Background: This study describes a strategy to select and isolate spontaneous riboflavin-overproducing strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and Propionibacterium (P.) freudenreichii. Results: The toxic riboflavin analogue roseoflavin was used to isolate natural riboflavin-overproducing variants of the food grade micro-organisms Lb. plantarum, Lc. mesenteroides and P. freudenreichii strains. The method was successfully employed for strains of all three species. The mutation(s) responsible for the observed overproduction of riboflavin were identified for isolates of two species. Conclusion: Selection for spontaneous roseoflavin-resistant mutants was found to be a reliable method to obtain natural riboflavin-overproducing strains of a number of species commonly used in the food industry. This study presents a convenient method for deriving riboflavin-overproducing strains of bacterial starter cultures, which are currently used in the food industry, by a non-recombinant methodology. Use of such starter strains can be exploited to increase the vitamin content in certain food products.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/254
http://dx.doi.org/10.1186/1475-2859-5-24
Appears in Collections:Food Safety
Teagasc publications in Biomed Central

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