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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/292

Title: Genetic merit for fertility traits in Holstein cows: III. Hepatic expression of somatotropic axis genes during pregnancy and lactation
Authors: Cummins, Sean B
Waters, Sinead M.
Evans, A.C.O.
Lonergan, P.
Butler, Stephen T.
Keywords: genetic merit for fertility
somatotropic axis
insulin-like growth factor-I
fertility
Issue Date: Jul-2012
Publisher: American Dairy Science Association and Elsevier Inc.
Citation: S.B. Cummins, S.M. Waters, A.C.O. Evans, P. Lonergan, S.T. Butler. Genetic merit for fertility traits in Holstein cows: III. Hepatic expression of somatotropic axis genes during pregnancy and lactation. Journal of Dairy Science, 95(7), July 2012: 3711-3721. DOI:10.3168/jds.2011-4977
Series/Report no.: Journal of Dairy Science;Vol. 95
Abstract: The objective of this study was to characterize the circulating concentrations of insulin-like growth factor-I (IGF-I) and the hepatic expression of key genes regulating the somatotropic axis in cows divergent in genetic merit for fertility traits but with similar genetic merit for milk production traits. A total of 11 cows with good genetic merit for fertility (Fert+) and 12 cows with poor genetic merit for fertility (Fert−) underwent liver biopsy by percutaneous punch technique on d 20 (± 6.7 d) prepartum and on d 2 (± 1.5 d), d 58 (± 3.7 d), d 145 (± 13 d), and d 245 (± 17.1 d) postpartum. Total RNA was isolated and the mRNA expression of growth hormone receptor (GHR 1A and GHRtot), IGF-I, janus tyrosine kinase 2 (JAK2), signal transducer and activator of transcription 5B (STAT5B), suppressor of cytokine signaling 3 (SOCS-3), acid-labile subunit (ALS), and IGF-binding proteins (IGFBP1 to IGFBP6) were measured by real-time quantitative PCR. During lactation, the circulating concentrations of IGF-I were 34% greater in Fert+ cows. The Fert+ cows had increased mean expression of IGF-I mRNA during the study; however, the difference in IGF-I mRNA abundance between Fert+ and Fert− cows was most pronounced at d 145 and 245. The expression of IGFBP3 and ALS transcript was similar in Fert+ and Fert− cows for the duration of the study. The Fert− cows, however, had greater expression of IGFBP2, IGFBP4, IGFBP5, and IGFBP6. Genotype had no effect on mRNA abundance of GHR 1A, STAT5B, JAK2, or SOCS-3. Genetic merit for fertility traits affects hepatic expression of key genes of the somatotropic axis regulating the synthesis, bioavailability, and stability of circulating IGF-I.
Description: Peer-reviewed
This is the author’s version of a work that was accepted for publication in Journal of Dairy Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Dairy Science, Volume 95, Issue 7, July 2012, Pages 3711–3721: DOI 10.3168/jds.2011-4977
URI: http://hdl.handle.net/11019/292
http://dx.doi.org/10.3168/jds.2011-4977
http://www.sciencedirect.com/science/article/pii/S0022030212003517
ISSN: 0022-0302
Appears in Collections:Animal & Bioscience

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