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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/454

Title: The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria
Authors: Draper, Lorraine A
Cotter, Paul D.
Hill, Colin
Ross, R Paul
Keywords: Antimicrobial
Synergy
Lantibiotic
Bacteriocin
Lacticin 3147
Polymyxin
Issue Date: 26-Sep-2013
Publisher: Biomed Central
Citation: Lorraine A Draper, Paul D Cotter, Colin Hill and R Paul Ross. The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria. BMC Microbiology, 2013, 13:212. DOI:10.1186/1471-2180-13-212
Series/Report no.: BMC Microbiology;vol 13
Abstract: Background: The emergence of bacterial drug resistance encourages the re-evaluation of the potential of existing antimicrobials. Lantibiotics are post-translationally modified, ribosomally synthesised antimicrobial peptides with a broad spectrum antimicrobial activity. Here, we focussed on expanding the potential of lacticin 3147, one of the most studied lantibiotics and one which possesses potent activity against a wide range of Gram positive species including many nosocomial pathogens. More specifically, our aim was to investigate if lacticin 3147 activity could be enhanced when combined with a range of different clinical antibiotics. Results: Initial screening revealed that polymyxin B and polymyxin E (colistin) exhibited synergistic activity with lacticin 3147. Checkerboard assays were performed against a number of strains, including both Gram positive and Gram negative species. The resultant fractional inhibitory concentration (FIC) index values established that, while partial synergy was detected against Gram positive targets, synergy was obvious against Gram negative species, including Cronobacter and E. coli. Conclusions: Combining lacticin 3147 with low levels of a polymyxin could provide a means of broadening target specificity of the lantibiotic, while also reducing polymyxin use due to the lower concentrations required as a result of synergy.
Description: peer-reviewed
peer-reviewed
URI: http://hdl.handle.net/11019/454
http://dx.doi.org/10.1186/1471-2180-13-212
ISSN: 1471-2180
Appears in Collections:Teagasc publications in Biomed Central
Food Biosciences

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