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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/480

Title: Bactofencin A, a New Type of Cationic Bacteriocin with Unusual Immunity
Authors: O'Shea, Eileen F.
O'Connor, Paula M.
O'Sullivan, Orla
Cotter, Paul D.
Ross, R Paul
Hill, Colin
Keywords: L.Salivarius
Bacteriocin
Bactofencin
Probiotic
Genome
Issue Date: 29-Oct-2013
Publisher: American Society for Microbiology
Citation: O’Shea EF, O’Connor PM, O’Sullivan O, Cotter PD, Ross RP, Hill C. 2013. Bactofencin A, a new type of cationic bacteriocin with unusual immunity. mBio 4(6):e00498-13. doi:10.1128/mBio.00498-13
Series/Report no.: MBio;vol 4
Abstract: Bacteriocin production is an important probiotic trait of intestinal bacteria. In this study, we identify a new type of bacteriocin, bactofencin A, produced by a porcine intestinal isolate Lactobacillus salivarius DPC6502, and assess its potency against pathogenic species including Staphylococcus aureus and Listeria monocytogenes. Genome sequencing of the bacteriocin producer revealed bfnA, which encodes the mature and highly basic (pI 10.59), 22-amino-acid defensin-like peptide. Matrixassisted laser desorption ionization–time of flight (MALDI-TOF) mass spectral analysis determined that bactofencin A has a molecular mass of 2,782 Da and contains two cysteine residues that form an intramolecular disulfide bond. Although an ABC transporter and transport accessory protein were also present within the bacteriocin gene cluster, a classical bacteriocin immunity gene was not detected. Interestingly, a dltB homologue was identified downstream of bfnA. DltB is usually encoded within the dlt operon of many Gram-positive bacteria. It is responsible for D-alanylation of teichoic acids in the cell wall and has previously been associated with bacterial resistance to cationic antimicrobial peptides. Heterologous expression of this gene conferred bactofencin A-specific immunity on sensitive strains of L. salivarius and S. aureus (although not L. monocytogenes), establishing its role in bacteriocin immunity. An analysis of the distribution of bfnA revealed that it was present in four additional isolates derived from porcine origin and absent from five human isolates, suggesting that its distribution is host specific. Given its novelty, we anticipate that bactofencin A represents the prototype of a new class of bacteriocins characterized as being cationic, with a DltB homologue providing a cognate immunity function.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/480
http://dx.doi.org/10.1128/mBio.00498-13
http://mbio.asm.org/content/4/6/e00498-13.full.pdf+html
Appears in Collections:Food Safety
Food Biosciences

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