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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/488

Title: Detection of banned nitrofuran metabolites in animal plasma samples using UHPLC–MS/MS
Authors: Radovnikovic, Anita
Moloney, Mary
Byrne, Patrick
Danaher, Martin
Keywords: Nitrofuran metabolites
Plasma
Residue determination
UHPLC–MS/MS
Stability study
Issue Date: 7-Dec-2010
Publisher: Elsevier
Citation: Anita Radovnikovic, Mary Moloney, Paddy Byrne, Martin Danaher. Detection of banned nitrofuran metabolites in animal plasma samples using UHPLC–MS/MS. Journal of Chromatography B, 2011, 879(2), 159-166. DOI: 10.1016/j.jchromb.2010.11.036.
Series/Report no.: Journal of Chromatography B;vol 879
Abstract: The use of nitrofurans as veterinary drugs in food-producing animals has been banned in the EU since the 1990s. Monitoring programs in the EU are based on the detection of protein-bound metabolites after slaughter. An UHPLC–MS/MS method was developed and validated for pre slaughter determination of four nitrofuran metabolites (AHD, AOZ, SEM, AMOZ) in animal plasma (bovine, ovine, equine and porcine). This method is proposed as an alternative method for on-farm surveillance. Plasma samples were derivatised with 2-nitrobenzaldehyde and subsequently extracted with organic solvent. Extracts were concentrated and then analysed by UHPLC–MS/MS. The method was validated according to Commission Decision 2002/657/EC. Inter-species recovery for AHD, AOZ, SEM and AMOZ was 72, 74, 57 and 71%, respectively. Decision limits (CC ) were calculated from within laboratory reproducibility experiments to be 0.070, 0.059, 0.071 and 0.054 gkg−1, respectively. In addition, the assay was applied to incurred plasma samples taken from pigs treated with furazolidone.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/488
http://dx.doi.org/10.1016/j.jchromb.2010.11.036
http://www.sciencedirect.com/science/article/pii/S1570023210007397
ISSN: 1570-0232
Appears in Collections:Food Safety

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