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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/489

Title: Benzimidazole carbamate residues in milk: Detection by Surface Plasmon Resonance-biosensor, using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method for extraction
Authors: Keegan, Jemma
Whelan, Michelle
Danaher, Martin
Crooks, Steven
Sayers, Riona
Anastasio, Aniello
Elliott, Chrtistopher
Brandon, David
Furey, Ambrose
O'Kennedy, Richard
Keywords: SPR-biosensors
Benzimidazole carbamates
Bovine milk
QuEChERS
Issue Date: 26-Sep-2009
Publisher: Elsevier
Citation: Jemma Keegan, Michelle Whelan, Martin Danaher, Steven Crooks, Regina Sayers, Aniello Anastasio, Christopher Elliott, David Brandon, Ambrose Furey, Richard O’Kennedy. Benzimidazole carbamate residues in milk: Detection by Surface Plasmon Resonance-biosensor, using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method for extraction. Analytica Chimica Acta, 2009, 654(2), 111-119. DOI: 10.1016/j.aca.2009.09.028.
Series/Report no.: Analytica Chimica Acta;vol 654
Abstract: A surface plasmon resonance (SPR) biosensor screening assay was developed and validated to detect 11 benzimidazole carbamate (BZT) veterinary drug residues in milk. The polyclonal antibody used was raised in sheep against a methyl 5(6)-[(carboxypentyl)-thio]-2-benzimidazole carbamate protein conjugate. A sample preparation procedure was developed using a modified QuEChERS method. BZT residues were extracted from milk using liquid extraction/partition with a dispersive solid phase extraction clean-up step. The assay was validated in accordance with the performance criteria described in 2002/657/EC. The limit of detection of the assay was calculated from the analysis of 20 known negative milk samples to be 2.7 μg kg−1. The detection capability (CCβ) of the assay was determined to be 5 μg kg−1 for 11 benzimidazole residues and the mean recovery of analytes was in the range 81–116%. A comparison was made between the SPR-biosensor and UPLC–MS/MS analyses of milk samples (n = 26) taken from cows treated different benzimidazole products, demonstrating the SPR-biosensor assay to be fit for purpose.
Description: peer-reviewed
This research was funded by the Irish Department of Agriculture, Fisheries and Food under the Food Institutional Research Measure as part of the National Development Plan.
URI: http://hdl.handle.net/11019/489
http://dx.doi.org/10.1016/j.aca.2009.09.028
http://www.sciencedirect.com/science/article/pii/S0003267009012963
ISSN: 0003-2670
Appears in Collections:Food Safety

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