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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/501

Title: Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture
Authors: Keane, Orla M
Budd, Kathleen E
Flynn, James
McCoy, Finola
Keywords: Polymerase Chain Reaction
Mastitis pathogens
Bacterial culture
Staphylococcus aureus
Issue Date: 23-Aug-2013
Publisher: British Veterinary Association
Citation: Keane O.M., Budd K.E., Flynn J. and McCoy F. Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture. Veterinary Record 2013;173:268 doi:10.1136/vr.101598
Series/Report no.: Veterinary Record;vol 173
Abstract: Rapid and accurate identification of mastitis pathogens is important for disease control. Bacterial culture and isolate identification is considered the gold standard in mastitis diagnosis but is time consuming and results in many culture-negative samples. Identification of mastitis pathogens by PCR has been proposed as a fast and sensitive alternative to bacterial culture. The results of bacterial culture and PCR for the identification of the aetiological agent of clinical mastitis were compared. The pathogen identified by traditional culture methods was also detected by PCR in 98 per cent of cases indicating good agreement between the positive results of bacterial culture and PCR. A mastitis pathogen could not be recovered from approximately 30 per cent of samples by bacterial culture, however, an aetiological agent was identified by PCR in 79 per cent of these samples. Therefore, a mastitis pathogen was detected in significantly more milk samples by PCR than by bacterial culture (92 per cent and 70 per cent, respectively) although the clinical relevance of PCR-positive culture-negative results remains controversial. A mixed infection of two or more mastitis pathogens was also detected more commonly by PCR. Culture-negative samples due to undetected Staphylococcus aureus infections were rare. The use of PCR technology may assist in rapid mastitis diagnosis, however, accurate interpretation of PCR results in the absence of bacterial culture remains problematic.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/501
http://dx.doi.org/10.1136/vr.101598
http://veterinaryrecord.bmj.com/content/173/11/268
ISSN: 0042-4900
Appears in Collections:Animal & Bioscience

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