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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/511

Title: Tryptophan-Mediated Denaturation of β-Lactoglobulin A by UV Irradiation
Authors: Kehoe, Joseph James
Remondetto, Gabriel E
Subirade, Muriel
Morris, Edwin R
Brodkorb, Andre
Keywords: β-Lactoglobulin
UV irradiation
Tryptophan
Disulfide
Photo-oxidation
Issue Date: 4-Jun-2008
Publisher: American Chemical Society
Citation: Kehoe, J. J., Remondetto, G. E., Subirade, M., Morris, E. R., & Brodkorb, A. (2008). Tryptophan-Mediated Denaturation of β-Lactoglobulin A by UV Irradiation. Journal of Agricultural and Food Chemistry, 56(12), 4720-4725. DOI: 10.1021/jf0733158
Series/Report no.: Journal of Agricultural and Food Chemistry;vol 56
Abstract: β-Lactoglobulin A, a genetic variant of one of the main whey proteins, was irradiated at 295 nm for 24 h. After irradiation, 18% of the protein was denatured (determined by reverse-phase chromatography). The fluorescence spectrum of the irradiated protein was red-shifted compared to that of the native protein, indicating a change in protein folding. Sulfhydryl groups, which are buried in native β-lactoglobulin, were exposed following irradiation and became available for quantification using the Ellman assay. The quantity of exposed sulfhydryls increased, but the number of total sulfhydryl groups decreased. Gel permeation chromatography showed that some protein aggregation occurred during irradiation. Fourier transform infrared (FTIR) spectroscopy of irradiated β-lactoglobulin revealed changes in the secondary structure, comparable to that of early events during heat-induced denaturation. There was evidence for some photo-oxidation of tryptophan.
Description: peer-reviewed
URI: http://hdl.handle.net/11019/511
http://dx.doi.org/10.1021/jf0733158
http://pubs.acs.org/doi/abs/10.1021/jf0733158
ISSN: 0021-8561
Appears in Collections:Food Chemistry & Technology

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