Crops, Environment & Land Use Programme >
Please use this identifier to cite or link to this item:
|Title: ||Effect of organic, conventional and mixed cultivation practices on soil microbial community structure and nematode abundance in a cultivated onion crop|
|Authors: ||Reilly, Kim|
Griffiths, Bryan S
|Keywords: ||Biolog Eco-plates|
Community level physiological profile
Denaturing gradient gel electrophoresis
|Issue Date: ||7-Jun-2013|
|Citation: ||Reilly, K., Cullen, E., Lola-Luz, T., Stone, D., Valverde, J., Gaffney, M., Brunton, N., Grant, J. and Griffiths, B. S. (2013), Effect of organic, conventional and mixed cultivation practices on soil microbial community structure and nematode abundance in a cultivated onion crop. J. Sci. Food Agric., 93: 3700–3709. doi: 10.1002/jsfa.6206|
|Series/Report no.: ||Journal of the Science of Food and Agriculture;vol 93|
|Abstract: ||BACKGROUND: Responses of the soil microbial and nematode community to organic and conventional agricultural practices were studied using the Teagasc Kinsealy Systems Comparison trial as the experimental system. The trial is a long term field experiment which divides conventional and organic agriculture into component pest-control and soil treatment practices. We hypothesised that management practices would affect soil ecology and used community level physiological profiles (CLPP), microbial and nematode counts, and denaturing gradient gel electrophoresis (DGGE) to characterise soil microbial communities in plots used for onion (Allium cepa L.) cultivation.
RESULTS: Microbial activity and culturable bacterial counts were significantly higher under fully organic management. Culturable fungi, actinomycete and nematode counts showed a consistent trend towards higher numbers under fully organic management but these data were not statistically significant. No differences were found in the fungal/bacterial ratio. DGGE banding patterns and sequencing of excised bands showed clear differences between treatments. Putative onion fungal pathogens were predominantly sequenced under conventional soil treatment practices whilst putative soil suppressive bacterial species were predominantly sequenced from the organic pest-control treatment plots.
CONCLUSION: Organic management increased microbial activity and diversity. Sequence data was indicative of differences in functional groups and warrants further investigation.|
|Description: ||pre-print, non-peer-reviewed|
The Irish Department of Agriculture, Fisheries and Food (FIRM 06/NITARFC6) is gratefully acknowledged for financial support. TLL thanks IRCSET and OGT for financial support.
|Appears in Collections:||Environment, Soils & Land Use|
Items in T-Stor are protected by copyright, with all rights reserved, unless otherwise indicated.