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Please use this identifier to cite or link to this item: http://hdl.handle.net/11019/579

Title: Insights from the Fungus Fusarium oxysporum Point to High Affinity Glucose Transporters as Targets for Enhancing Ethanol Production from Lignocellulose
Authors: Ali, Shahin S
Nugent, Brian
Mullins, Ewen
Doohan, Fiona M.
Keywords: Biofuels
Fusarium oxysporum
Fermentation
Straw
Microbial bioprocessing
Glucose
Xylose
Wheat
Ethanol
Membrane proteins
Yeast
Issue Date: 30-Jan-2013
Publisher: PLOS
Citation: Ali SS, Nugent B, Mullins E, Doohan FM (2013) Insights from the Fungus Fusarium oxysporum Point to High Affinity Glucose Transporters as Targets for Enhancing Ethanol Production from Lignocellulose. PLoS ONE 8(1): e54701. doi:10.1371/journal.pone.0054701
Series/Report no.: PLOS ONE;
Abstract: Ethanol is the most-widely used biofuel in the world today. Lignocellulosic plant biomass derived from agricultural residue can be converted to ethanol via microbial bioprocessing. Fungi such as Fusarium oxysporum can simultaneously saccharify straw to sugars and ferment sugars to ethanol. But there are many bottlenecks that need to be overcome to increase the efficacy of microbial production of ethanol from straw, not least enhancement of the rate of fermentation of both hexose and pentose sugars. This research tested the hypothesis that the rate of sugar uptake by F. oxysporum would enhance the ethanol yields from lignocellulosic straw and that high affinity glucose transporters can enhance ethanol yields from this substrate. We characterized a novel hexose transporter (Hxt) from this fungus. The F. oxysporum Hxt represents a novel transporter with homology to yeast glucose signaling/transporter proteins Rgt2 and Snf3, but it lacks their C-terminal domain which is necessary for glucose signalling. Its expression level decreased with increasing glucose concentration in the medium and in a glucose uptake study the Km(glucose) was 0.9 mM, which indicated that the protein is a high affinity glucose transporter. Post-translational gene silencing or over expression of the Hxt in F. oxysporum directly affected the glucose and xylose transport capacity and ethanol yielded by F. oxysporum from straw, glucose and xylose. Thus we conclude that this Hxt has the capacity to transport both C5 and C6 sugars and to enhance ethanol yields from lignocellulosic material. This study has confirmed that high affinity glucose transporters are ideal candidates for improving ethanol yields from lignocellulose because their activity and level of expression is high in low glucose concentrations, which is very common during the process of consolidated processing.
Description: peer-reviewed
This work was supported by the Irish Department of Agriculture, Fisheries & Food Research Stimulus Fund (RSF 07 513).
URI: http://hdl.handle.net/11019/579
http://dx.doi.org/10.1371/journal.pone.0054701
ISSN: 1932-6203
Appears in Collections:Crop Science

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