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  • PublicationOpen Access
    Incorporating a Fresh Mixed Annual Ryegrass and Berseem Clover Forage Into the Winter Diet of Dairy Cows Resulted in Reduced Milk Yield, but Reduced Nitrogen Excretion and Reduced Methane Yield
    (Frontiers Media SA, 2020-11-20) Enriquez-Hidalgo, Daniel; Teixeira, Dayane Lemos; Pinheiro Machado Filho, Luiz Carlos; Hennessy, Deirdre; Toro-Mujica, Paula; Williams, Shaun Richard Owen; Pereira, Fabiellen Cristina; National Commission for Scientific and Technological Research; 11160697
    The winter diet of dairy cows in Mediterranean climate regions is usually a total mixed ration with a base of conserved summer crops such as corn silage and alfalfa hay. However, there is increased labor and financial cost related to this kind of feeding, which could be reduced if fresh forages were used in place of some of the conserved forage in the cow diet. The objective of our study was to evaluate the effect of including fresh mixed annual ryegrass and berseem clover into the diet of dairy cows on milk, nitrogen utilization, and methane emission. Twenty-four lactating dairy cows were split into two groups and offered either a diet similar to that usually offered to the cows (CON) or one where a mixture of fresh annual ryegrass and berseem clover was used to partially substitute the corn silage and alfalfa hay in the diet (MIX). Milk yield was recorded automatically, and methane emissions were estimated using the SF6 tracer technique. The MIX diet had lower crude protein concentration (148 vs. 170 g/kg DM) but higher DM digestibility (81.6 vs. 78.6%) than the CON diet. Compared to the cows offered the CON diet, milk yield was reduced when cows were fed the MIX diet (36.4 vs. 31.9 kg/d), but methane emissions (381 vs. 332 g/d) and nitrogen excretion were also reduced (238 vs. 180 g/d). Nitrogen use efficiency was unaffected (30.8%). In addition, milk from cows fed the MIX diet had a fatty acid profile considered to be more beneficial to human health than that of the milk from cows fed the CON diet. Increasing the protein concentration in the MIX diet, either by direct supplementation or increasing the proportion of legume in the mixed herbage, could overcome the reduction on milk and positively affect methane emission and N use efficiency.
  • ItemOpen Access
    Phytochemicals of Minthostachys diffusa Epling and Their Health-Promoting Bioactivities
    (MDPI AG, 2020-2-1) Faraone, Immacolata; Russo, Daniela; Chiummiento, Lucia; Fernandez, Eloy; Choudhary, Alka; Monné, Magnus; Milella, Luigi; Rai, Dilip K.; Regione Basilicata
    The genus Minthostachys belonging to the Lamiaceae family, and is an important South American mint genus used commonly in folk medicine as an aroma in cooking. The phytochemical-rich samples of the aerial parts of Minthostachys diffusa Epling. were tested for pharmacological and health-promoting bioactivities using in vitro chemical and enzymatic assays. A range of radical scavenging activities of the samples against biological radicals such as nitric oxide and superoxide anion and against synthetic 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, the ferric reducing antioxidant power and the lipid peroxidation inhibition were determined and ranked using the ‘relative antioxidant capacity index’ (RACI). The ethyl acetate fraction showed the highest RACI of +1.12. Analysis of the various fractions’ inhibitory ability against enzymes involved in diabetes (α-amylase and α-glucosidase), and against enzymes associated with Parkinson’s or Alzheimer’s diseases (acetylcholinesterase and butyrylcholinesterase) also suggested that the ethyl acetate fraction was the most active. Liquid chromatography–tandem mass spectrometry analysis of the ethyl acetate fraction showed more than 30 polyphenolic compounds, including triterpenes. The inhibitory cholinesterase effects of the triterpenes identified from M. diffusa were further analysed by in silico docking of these compounds into 3D-structures of acetylcholinesterase and butyrylcholinesterase. This is the first study on pharmacological activities and phytochemical profiling of the aerial parts of M. diffusa, showing that this plant, normally used as food in South America, is also rich in health-promoting phytochemicals.
  • PublicationOpen Access
    The Impact of Hogget and Mature Flock Reproductive Success on Sheep Farm Productivity
    (MDPI AG, 2020-11-21) Farrell, Lydia J.; Kenyon, Paul R.; Morris, Stephen T.; Tozer, Peter R.; Massey University
    Breeding hoggets (ewe lambs aged four to 16 months) at 8 to 9 months of age has a number of potential benefits, including increased lamb production and profitability. However, the majority of hoggets in New Zealand are not bred due to producer concerns regarding their variable reproductive success and increased feed demand. Simulation modelling was used to quantify effects of hogget breeding on sheep numbers, lamb production, sheep feed demand, and sheep enterprise cash operating surplus (COS) compared with a flock not breeding hoggets. Hogget weaning rate (HWR) was modelled at 0%, 60%, 80%, and 100% and combined with mature ewe flock weaning rates (FWR) of 132% and 150%, while maintaining total annual sheep feed demand. For each FWR, increased HWR reduced total sheep numbers, increased the proportion of sheep feed demand for lamb production, increased total numbers of lambs weaned, and increased COS. Therefore, achieving even a relatively low HWR of 60% can improve sheep enterprise profitability for a given FWR. However, COS was lower with FWR = 132% and HWR = 100% than with FWR = 150% and HWR = 0%. The results indicate farmers who do not currently breed their hoggets may wish to improve their FWR before considering HWRs.
  • ItemOpen Access
    Can methyl jasmonate treatment of conifer seedlings be used as a tool to stop height growth in nursery forest trees?
    (Springer Science and Business Media LLC, 2019-8-12) Fedderwitz, Frauke; Björklund, Niklas; Anngren, Ronny; Lindström, Anders; Nordlander, Göran
    A plant's induced defense system can be triggered by the application of the plant hormone methyl jasmonate (MeJA), and recent research suggest that MeJA treatment may become a tool for protection of conifer seedlings against insect herbivory (e.g. by the pine weevil Hylobius abietis). A side-effect of MeJA application is temporarily reduced height growth. This has generally been considered as negative, but in forest tree nurseries this could instead be beneficial since it is commonly desired to stop the growth of nursery seedlings in late summer. Artificially longer dark periods (long nights/short days) are widely used in high-latitude nurseries to terminate height growth and induce freezing tolerance. However, long night treatment requires specialized nursery equipment and are labor intensive. Therefore alternatives are sought after. We compared long-night and MeJA treatments by following the growth of Norway spruce (Picea abies) seedlings throughout one season. The regulatory effect of MeJA on height growth was similar if not even better than that of long nights, i.e. it was terminated faster. However, MeJA treatment also reduced root growth and delayed the development of freezing tolerance. MeJA may therefore not replace long-night treatments, but it could facilitate a more flexible application of long nights by gaining a longer time interval during which this treatment can be used without risking the seedlings growing too large.
  • ItemOpen Access
    Replacing fishmeal with plant protein in Atlantic salmon (Salmo salar) diets by supplementation with fish protein hydrolysate
    (Springer Science and Business Media LLC, 2020-3-6) Egerton, S.; Wan, A.; Murphy, K.; Collins, F.; Ahern, G.; Sugrue, Katie; Busca, K.; Egan, F.; Muller, N.; Whooley, J.; McGinnity, P.; Culloty, S.; Ross, R. Paul; Stanton, Catherine; Science Foundation Ireland; SFI/12/RC/2273
    AbstractThe effects of feeding an 80% plant protein diet, with and without fish protein hydrolysate (FPH) supplementation, on the growth and gut health of Atlantic salmon were investigated. Fish were fed either (A) a control diet containing 35% fishmeal, (B) an 80% plant protein diet with 15% fishmeal, (C) an 80% plant protein diet with 5% fishmeal and 10% partly hydrolysed protein, or (D) an 80% plant protein diet with 5% fishmeal and 10% soluble protein hydrolysate. Fish on the 80% plant- 15% fishmeal diet were significantly smaller than fish in the other dietary groups. However, partly-hydrolysed protein supplementation allowed fish to grow as well as fish fed the control 35% fishmeal diet. Fish fed the FPH diets (diets C and D) had significantly higher levels of amino acids in their blood, including 48% and 27% more branched chain amino acids compared to fish on the 35% fishmeal diet, respectively. Plant protein significantly altered gut microbial composition, significantly decreasing α-diversity. Spirochaetes and the familiesMoritellaceae, Psychromonadaceae, HelicobacteraceaeandBacteroidaceaewere all found at significantly lower abundances in the groups fed 80% plant protein diets compared to the control fishmeal diet.

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