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  • ItemOpen Access
    DATAMAN: A global database of nitrous oxide and ammonia emission factors for excreta deposited by livestock and land‐applied manure
    (Wiley, 2021-1-22) Beltran, Ignacio; van der Weerden, Tony J.; Alfaro, Marta A.; Amon, Barbara; de Klein, Cecile A. M.; Grace, Peter; Hafner, Sasha; Hassouna, Mélynda; Hutchings, Nicholas; Krol, Dominika J.; Leytem, April B.; Noble, Alasdair; Salazar, Francisco; Thorman, Rachel E.; Velthof, Gerard L.; UK Department for Environment, Food and Rural Affairs; New Zealand Government
    Nitrous oxide (N2O), ammonia (NH3), and methane (CH4) emissions from the manure management chain of livestock production systems are important contributors to greenhouse gases (GHGs) and NH3 emitted by human activities. Several studies have evaluated manure‐related emissions and associated key variables at regional, national, or continental scales. However, there have been few studies focusing on the drivers of these emissions using a global dataset. An international project was created (DATAMAN) to develop a global database on GHG and NH3 emissions from the manure management chain (housing, storage, and field) to identify key variables influencing emissions and ultimately to refine emission factors (EFs) for future national GHG inventories and NH3 emission reporting. This paper describes the “field” database that focuses on N2O and NH3 EFs from land‐applied manure and excreta deposited by grazing livestock. We collated relevant information (EFs, manure characteristics, soil properties, and climatic conditions) from published peer‐reviewed research, conference papers, and existing databases. The database, containing 5,632 observations compiled from 184 studies, was relatively evenly split between N2O and NH3 (56 and 44% of the EF values, respectively). The N2http://www.dataman.co.nz
  • ItemOpen Access
    Low-Molecular-Weight Seaweed-Derived Polysaccharides Lead to Increased Faecal Bulk but Do Not Alter Human Gut Health Markers
    (MDPI AG, 2021-12-3) Bannon, Ciara D.; Eckenberger, Julia; Snelling, William John; Huseyin, Chloe Elizabeth; Allsopp, Philip; Strain, Conall; Ramnani, Priya; Chitarrari, Roberto; Grant, John; Hotchkiss, Sarah; Philp, Kevin; Campbell, Ross; Tuohy, Kieran Michael; Claesson, Marcus J.; Ternan, Nigel George; Dooley, James S. G.; Sleator, Roy D.; Rowland, Ian; Gill, Chris I. R.; European Union Seventh Framework Programme|222470
    Seaweeds are potentially sustainable crops and are receiving significant interest because of their rich bioactive compound content; including fatty acids, polyphenols, carotenoids, and complex polysaccharides. However, there is little information on the in vivo effects on gut health of the polysaccharides and their low-molecular-weight derivatives. Herein, we describe the first investigation into the prebiotic potential of low-molecular-weight polysaccharides (LMWPs) derived from alginate and agar in order to validate their in vivo efficacy. We conducted a randomized; placebo-controlled trial testing the impact of alginate and agar LWMPs on faecal weight and other markers of gut health and on composition of gut microbiota. We show that these LMWPs led to significantly increased faecal bulk (20–30%). Analysis of gut microbiome composition by sequencing indicated no significant changes attributable to treatment at the phylum and family level, although FISH analysis showed an increase in Faecalibacterium prausnitzii in subjects consuming agar LMWP. Sequence analysis of gut bacteria corroborated with the FISH data, indicating that alginate and agar LWMPs do not alter human gut microbiome health markers. Crucially, our findings suggest an urgent need for robust and rigorous human in vivo testing—in particular, using refined seaweed extracts.
  • ItemOpen Access
    Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?
    (American Society for Microbiology, 2021-9-3) Baffoni, Loredana; Alberoni, Daniele; Gaggìa, Francesca; Braglia, Chiara; Stanton, Catherine; Ross, Paul R.; Di Gioia, Diana; European Union Horizon 2020|GA 777760
    This study investigates the impact of the three most widely used antibiotics in the beekeeping sector (oxytetracycline, tylosin, and sulfonamides) on the honeybee gut microbiota and on the spread of antibiotic resistance genes. The research represents an advance to the present literature, considering that the tylosin and sulfonamides effects on the gut microbiota have never been studied.
  • PublicationOpen Access
    Dairy Producers Who Market Their Surplus Progeny as Calves Use Germplasm With Slightly Lighter and Less-Conformed Carcasses Than Producers Who Rear Their Surplus Progeny Beyond Weaning
    (Frontiers Media SA, 2021-10-13) Berry, Donagh P.; Ring, Siobhan R.; Science Foundation Ireland|16/RC/3835; Department of Agriculture, Food and Marine
    Understanding dairy producer mindset in service sire selection can provide useful information for different junctures along the commercial and extension animal breeding chain. It can aid the targeted marketing of bulls based on farm production systems but also provide useful information for delivering bespoke extension services. The objective of the present study was to examine if differences exist among dairy producers in their choice of dairy and beef service sires depending on the life stage at which the surplus progeny generated from such matings exit the dairy farm. This was predominantly based on evaluating the breed of beef sires used but also their genetic merit for calving difficulty and carcass traits, namely, carcass weight, conformation, and fat score; differences in genetic merit among dairy sires as well as among the dairy cows themselves were also considered. The objective was accomplished through the cross-sectional analyses of progeny fate data from 1,092,403 progeny born in 4,117 Irish dairy herds. Herd-years were categorized into one of four systems based on when the surplus progeny exited the dairy farm: (1) calves sold <70 days of age, (2) cattle sold as yearlings between 250 and 450 days of age, (3) prime cattle sold for finishing (slaughtered between 8 and 120 days of exiting the dairy farm), or (4) prime cattle sold for immediate slaughter (i.e., slaughtered within 7 days of exiting the dairy farm). The mean genetic merit of both the cows and service sires used across the four different systems was estimated using linear mixed models. Of the beef service sires used in herds that sold their surplus progeny as calves, their mean predicted transmitting ability for carcass weight and carcass conformation score was just 2.00 kg and 0.11 scores [scale of 1 (poor) to 15 (excellent)] inferior to the beef service sires used in herds that sold their surplus progeny as prime cattle for immediate slaughter. Similar trends, albeit of smaller magnitude, were evident when comparing the genetic merit of the dairy service sires used in those systems. Cows in herds that sold their surplus progeny as calves were genetically less likely to incur dystocia as well as to have lighter, less-conformed, and leaner carcasses than cows in herds that sold their surplus progeny post-weaning. Hence, results from the present study suggest that diversity in herd strategy regarding when surplus progeny exit the herd influences service sire selection choices in respect of genetic merit for dystocia and carcass attributes. That said, the biological difference based on the current pool of available service sires is small relative to the dairy producers that sell their surplus progeny as young calves; when expressed on a per standard deviation in genetic merit of the beef service sires used across all herds, the difference between extreme systems was, nonetheless, approximately half a standard deviation for carcass weight and conformation.
  • ItemOpen Access
    Anticholinesterase Activities of Different Solvent Extracts of Brewer’s Spent Grain
    (MDPI AG, 2021-4-23) Birsan, Rares I.; Wilde, Peter; Waldron, Keith W.; Rai, Dilip K.; Teagasc Walsh Fellowship|2014027
    Cholinesterases, involved in acetylcholine catabolism in the central and peripheral nervous system, have been strongly linked with neurodegenerative diseases. Current therapeutic approaches using synthetic drugs present several side effects. Hence, there is an increasing research interest in naturally-occurring dietary polyphenols, which are also considered efficacious. Food processing by-products such as brewer’s spent grain (BSG) would be a potential bio-source of polyphenols. In this study, polyphenol-rich BSG extracts using 60% acetone and 0.75% NaOH solutions were generated, which were further subjected to liquid–liquid partitioning using various organic solvents. The water-partitioned fractions of the saponified extracts had the highest total polyphenol content (6.2 ± 2.8 mgGAE/g dw) as determined by Folin–Ciocalteu reagent, while the LC-MS/MS showed ethyl acetate fraction with the highest phenolics (2.9 ± 0.3 mg/g BSG dw). The best inhibitions of acetyl- (37.9 ± 2.9%) and butyryl- (53.6 ± 7.7%) cholinesterases were shown by the diethyl ether fraction of the saponified extract. This fraction contained the highest sum of quantified phenolics (99 ± 21.2 µg/mg of extract), and with significant (p < 0.01) inhibitory contribution of decarboxylated-diferulic acid. Amongst the standards, caffeic acid presented the highest inhibition for both cholinesterases, 25.5 ± 0.2% for acetyl- and 52.3 ± 0.8% for butyryl-cholinesterase, respectively, whilst the blends insignificantly inhibited both cholinesterases. The results showed that polyphenol-rich BSG fractions have potentials as natural anti-cholinesterase agents.

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