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Hilde K. L. Wærp, Sinéad M. Waters, Matthew S. McCabe, Paul Cormican, Ragnar Salte
RNA-seq analysis of bovine adipose tissue in heifers fed diets differing in energy and protein content
www.plosone.org
www.plosone.org
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1.0374 0 Td
(the)Tj
-35.0019 -1.2983 Td
(pre-)Tj
1.8821 0 Td
(and)Tj
1.7292 0 Td
(post-pubertal)Tj
5.5785 0 Td
(periods)Tj
3.1975 0 Td
(for)Tj
1.3833 0 Td
(a)Tj
0.6576 0 Td
(replacement)Tj
5.1477 0 Td
(dairy)Tj
2.2734 0 Td
(heifer.)Tj
2.7496 0 Td
(Eighty)Tj
2.8006 0 Td
(heifers)Tj
2.8799 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
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(dairy)Tj
-33.9588 -1.2983 Td
(herd)Tj
2.0692 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.4683 0 Td
(Norwegian)Tj
4.6601 0 Td
(University)Tj
4.3994 0 Td
(of)Tj
1.0148 0 Td
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1.7404 0 Td
(Sciences)Tj
3.566 0 Td
(\(years)Tj
2.6022 0 Td
(2010)Tj
2.1203 0 Td
(and)Tj
1.7348 0 Td
(2011\))Tj
2.4604 0 Td
(were)Tj
2.1203 0 Td
(assigned)Tj
3.6113 0 Td
(either)Tj
-34.52 -1.3039 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.6519 0 Td
(high)Tj
2.007 0 Td
(\(HE\))Tj
2.2393 0 Td
(or)Tj
1.0942 0 Td
(low)Tj
1.6611 0 Td
(\(LE\))Tj
2.0069 0 Td
(energy)Tj
2.9083 0 Td
(group,)Tj
2.8347 0 Td
(fed)Tj
1.457 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0205 0 Td
(a)Tj
0.6576 0 Td
(BW)Tj
1.7632 0 Td
(gain)Tj
1.9389 0 Td
(of)Tj
1.0148 0 Td
(8001000)Tj
4.0648 0 Td
(or)Tj
1.0998 0 Td
(600750)Tj
-33.6356 -1.2982 Td
(g/day)Tj
2.4321 0 Td
(from)Tj
2.2053 0 Td
(three)Tj
2.262 0 Td
(months)Tj
3.2825 0 Td
(of)Tj
1.0148 0 Td
(age)Tj
1.5421 0 Td
(to)Tj
1.0261 0 Td
(confirmed)Tj
4.3993 0 Td
(pregnancy,)Tj
4.6318 0 Td
(respectively.)Tj
5.108 0 Td
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(energy)Tj
2.9027 0 Td
(group)Tj
2.6078 0 Td
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(further)Tj
3.0273 0 Td
(subdivided)Tj
4.6091 0 Td
(into)Tj
1.8425 0 Td
(two)Tj
1.7122 0 Td
(protein)Tj
3.1521 0 Td
(groups,)Tj
3.1918 0 Td
(i.e.)Tj
1.3606 0 Td
(low)Tj
1.6611 0 Td
(\(LP\))Tj
1.9955 0 Td
(or)Tj
1.0999 0 Td
(high)Tj
2.0069 0 Td
(\(HP\))Tj
2.228 0 Td
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1.0205 0 Td
(give)Tj
1.8312 0 Td
(four)Tj
1.9162 0 Td
(dietary)Tj
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(treatments)Tj
4.4957 0 Td
(with)Tj
1.9955 0 Td
(20)Tj
1.1736 0 Td
(animals)Tj
3.3278 0 Td
(in)Tj
1.0375 0 Td
(each)Tj
2.0296 0 Td
(group,)Tj
/F12 1 Tf
2.8346 0 Td
[()-13()]TJ
/F6 1 Tf
1.3323 0 Td
(low-protein)Tj
4.9436 0 Td
(high-energy)Tj
5.04 0 Td
(\(LPHE\),)Tj
3.5603 0 Td
(high-protein)Tj
-31.7705 -1.2983 Td
(high-energy)Tj
5.0456 0 Td
(\(HPHE\),)Tj
3.7871 0 Td
(low-protein)Tj
4.9435 0 Td
(low-energy)Tj
4.6942 0 Td
(\(LPLE\))Tj
3.1011 0 Td
(and)Tj
1.7348 0 Td
(high-protein)Tj
5.2894 0 Td
(low-energy)Tj
4.6941 0 Td
(\(HPLE\).)Tj
-33.2898 -1.2983 Td
(The)Tj
1.7801 0 Td
(university)Tj
4.1952 0 Td
(herd)Tj
2.0693 0 Td
(consists)Tj
3.3506 0 Td
(of)Tj
1.0148 0 Td
(two)Tj
1.7064 0 Td
(genetic)Tj
3.0727 0 Td
(groups,)Tj
3.1918 0 Td
(one)Tj
1.7008 0 Td
(group)Tj
2.6079 0 Td
(with)Tj
1.9955 0 Td
(high)Tj
2.007 0 Td
(milk-yield)Tj
4.3199 0 Td
(\(HMY\))Tj
-33.012 -1.3039 Td
(and)Tj
1.7291 0 Td
(one)Tj
1.7007 0 Td
(group)Tj
2.6079 0 Td
(with)Tj
1.9956 0 Td
(low)Tj
1.6554 0 Td
(occurrence)Tj
4.6658 0 Td
(of)Tj
1.0148 0 Td
(clinical)Tj
3.0784 0 Td
(mastitis)Tj
3.3222 0 Td
(\(LCM\))Tj
2.999 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.075 0 Td
(As)Tj
1.2642 0 Td
(this)Tj
1.6724 0 Td
(genetic)Tj
3.0728 0 Td
(difference)Tj
-32.8533 -1.2982 Td
(could)Tj
2.4434 0 Td
(affect)Tj
2.3697 0 Td
(several)Tj
2.9311 0 Td
(variables)Tj
3.7133 0 Td
(of)Tj
1.0091 0 Td
(interest)Tj
3.2032 0 Td
(to)Tj
1.0204 0 Td
(our)Tj
1.6214 0 Td
(experiment,)Tj
5.0003 0 Td
(it)Tj
0.7767 0 Td
(was)Tj
1.6951 0 Td
(taken)Tj
2.4151 0 Td
(into)Tj
1.8369 0 Td
(account)Tj
3.3788 0 Td
(when)Tj
2.3981 0 Td
(allo-)Tj
-35.8126 -1.2983 Td
(cating)Tj
2.6532 0 Td
(heifers)Tj
2.8856 0 Td
(to)Tj
1.0261 0 Td
(dietary)Tj
2.9934 0 Td
(treatments)Tj
4.4957 0 Td
(to)Tj
1.0262 0 Td
(generate)Tj
3.6113 0 Td
(groups)Tj
2.965 0 Td
(which)Tj
2.6532 0 Td
(were)Tj
2.1203 0 Td
(balanced)Tj
3.7644 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
-34.384 -1.3039 Td
(genetic)Tj
3.067 0 Td
(group.)Tj
2.8346 0 Td
(Apart)Tj
2.5285 0 Td
(from)Tj
2.2054 0 Td
(this)Tj
1.6724 0 Td
(consideration,)Tj
5.93 0 Td
(heifers)Tj
2.88 0 Td
(were)Tj
2.126 0 Td
(randomly)Tj
4.1272 0 Td
(allocated)Tj
3.7587 0 Td
(to)Tj
1.0262 0 Td
(treatment)Tj
-32.156 -1.2983 Td
(group.)Tj
2.8346 0 Td
(Body)Tj
2.2847 0 Td
(weights)Tj
3.2485 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(])Tj
1.8538 0 Td
(were)Tj
2.1203 0 Td
(measured)Tj
4.1102 0 Td
(every)Tj
2.3471 0 Td
(second)Tj
3.0104 0 Td
(week,)Tj
2.4718 0 Td
(and)Tj
1.7348 0 Td
(body)Tj
2.211 0 Td
(condition)Tj
4.1159 0 Td
(scores)Tj
2.6702 0 Td
(\(BCS\))Tj
-35.0133 -1.2982 Td
(and)Tj
1.7291 0 Td
(height)Tj
2.7269 0 Td
(at)Tj
0.9524 0 Td
(withers)Tj
3.1578 0 Td
(\(WH\))Tj
2.6305 0 Td
(recorded)Tj
3.7984 0 Td
(every)Tj
2.3471 0 Td
(month)Tj
2.9197 0 Td
(for)Tj
1.3889 0 Td
(all)Tj
1.1452 0 Td
(experimental)Tj
5.4595 0 Td
(animals.)Tj
3.5546 0 Td
(The)Tj
1.7802 0 Td
(Norwe-)Tj
-33.5903 -1.2983 Td
(gian)Tj
1.9332 0 Td
(15)Tj
1.6894 0 Td
(scale)Tj
2.109 0 Td
(BCS)Tj
1.9389 0 Td
(system)Tj
2.9366 0 Td
(is)Tj
0.8504 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2756 0 Td
(Edmonson)Tj
4.6091 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1225 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(])Tj
1.8539 0 Td
(but)Tj
1.5533 0 Td
(calibrated)Tj
4.1499 0 Td
(for)Tj
1.389 0 Td
(Norwegian)Tj
4.6545 0 Td
(Red)Tj
-35.4782 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
/F11 1 Tf
1.1962 -1.2983 Td
(Heifer)Tj
2.8913 0 Td
(feeding)Tj
3.2938 0 Td
(and)Tj
1.7972 0 Td
(management.)Tj
/F6 1 Tf
6.4686 0 Td
(All)Tj
1.3946 0 Td
(heifer)Tj
2.5229 0 Td
(calves)Tj
2.5625 0 Td
(were)Tj
2.1203 0 Td
(fed)Tj
1.4626 0 Td
(the)Tj
1.4684 0 Td
(same)Tj
2.2563 0 Td
(diet)Tj
1.7405 0 Td
(from)Tj
2.2053 0 Td
(birth)Tj
2.1884 0 Td
(to)Tj
-35.5689 -1.2983 Td
(three)Tj
2.262 0 Td
(mo)Tj
1.5477 0 Td
(of)Tj
1.0148 0 Td
(age.)Tj
1.7688 0 Td
(From)Tj
2.4434 0 Td
(three)Tj
2.2677 0 Td
(months)Tj
3.2825 0 Td
(of)Tj
1.0148 0 Td
(age)Tj
1.5421 0 Td
(until)Tj
2.1089 0 Td
(confirmed)Tj
4.3937 0 Td
(pregnancy,)Tj
4.6318 0 Td
(heifers)Tj
2.88 0 Td
(were)Tj
2.1259 0 Td
(housed)Tj
3.0955 0 Td
(in)Tj
-36.3796 -1.3039 Td
(a)Tj
0.6519 0 Td
(tie-stall)Tj
3.1465 0 Td
(barn)Tj
2.0806 0 Td
(and)Tj
1.7291 0 Td
(fed)Tj
1.4627 0 Td
(one)Tj
1.6951 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(four)Tj
1.9162 0 Td
(experimental)Tj
5.4595 0 Td
(diets.)Tj
2.3244 0 Td
(Fresh)Tj
2.4264 0 Td
(feed)Tj
1.8879 0 Td
(was)Tj
1.6951 0 Td
(offered)Tj
3.0501 0 Td
(twice)Tj
2.3187 0 Td
(daily)Tj
-34.3273 -1.2982 Td
(and)Tj
1.7291 0 Td
(individual)Tj
4.2859 0 Td
(feed)Tj
1.8822 0 Td
(intake)Tj
2.6929 0 Td
(was)Tj
1.6951 0 Td
(recorded)Tj
3.7984 0 Td
(four)Tj
1.9106 0 Td
(days)Tj
2.0012 0 Td
(a)Tj
0.652 0 Td
(week.)Tj
2.4718 0 Td
(HE)Tj
1.559 0 Td
(groups)Tj
2.9707 0 Td
(were)Tj
2.1203 0 Td
(fed)Tj
1.457 0 Td
(grass)Tj
2.2167 0 Td
(silage)Tj
2.4151 0 Td
(ad)Tj
-35.858 -1.2983 Td
(libitum)Tj
3.1521 0 Td
(whereas)Tj
3.4469 0 Td
(LE)Tj
1.3266 0 Td
(groups)Tj
2.9707 0 Td
(were)Tj
2.1203 0 Td
(fed)Tj
1.457 0 Td
(the)Tj
1.474 0 Td
(same)Tj
2.2563 0 Td
(silage)Tj
2.4151 0 Td
(mixed)Tj
2.7269 0 Td
(with)Tj
1.9956 0 Td
(10)Tj
1.1679 0 Td
(to)Tj
1.0261 0 Td
(40%)Tj
1.9276 0 Td
(wheat)Tj
2.5908 0 Td
(straw)Tj
2.3698 0 Td
(\(on)Tj
1.6157 0 Td
(a)Tj
-36.0394 -1.3039 Td
(dry)Tj
1.5703 0 Td
(matter)Tj
2.8687 0 Td
(basis\))Tj
2.4888 0 Td
(given)Tj
2.3811 0 Td
(as)Tj
1.0148 0 Td
(restricted)Tj
3.9855 0 Td
(rations.)Tj
3.2314 0 Td
(All)Tj
1.3947 0 Td
(heifers)Tj
2.8856 0 Td
(were)Tj
2.1203 0 Td
(daily)Tj
2.1487 0 Td
(fed)Tj
1.4626 0 Td
(1)Tj
0.6917 0 Td
(kg)Tj
1.1849 0 Td
(of)Tj
1.0148 0 Td
(either)Tj
2.5284 0 Td
(of)Tj
1.0148 0 Td
(two)Tj
1.7122 0 Td
(cus-)Tj
-35.6993 -1.2983 Td
(tom-made)Tj
4.3993 0 Td
(concentrates,)Tj
5.5048 0 Td
(differing)Tj
3.668 0 Td
(in)Tj
1.0375 0 Td
(protein)Tj
3.1521 0 Td
(content.)Tj
3.4923 0 Td
(Energy)Tj
3.05 0 Td
(density)Tj
3.1011 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(diets)Tj
2.0977 0 Td
(was)Tj
1.6951 0 Td
(adjusted)Tj
-33.6867 -1.2983 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.4684 0 Td
(roughage)Tj
3.9458 0 Td
(quality.)Tj
3.1804 0 Td
(Diet)Tj
1.9446 0 Td
(protein)Tj
3.1521 0 Td
(content)Tj
3.2655 0 Td
(was)Tj
1.7007 0 Td
(adjusted)Tj
3.5773 0 Td
(with)Tj
1.9956 0 Td
(both)Tj
2.058 0 Td
(roughage)Tj
3.9458 0 Td
(quality)Tj
2.9537 0 Td
(and)Tj
-35.1834 -1.3039 Td
(type)Tj
1.9105 0 Td
(of)Tj
1.0148 0 Td
(concentrate.)Tj
0.83 0.64 0.02 0 k
5.1477 0 Td
(Table)Tj
2.4434 0 Td
(1)Tj
0 g
0.6917 0 Td
(gives)Tj
2.194 0 Td
(the)Tj
1.4683 0 Td
(average)Tj
3.2258 0 Td
(nutrient)Tj
3.5036 0 Td
(content)Tj
3.2712 0 Td
(of)Tj
1.0148 0 Td
(roughage)Tj
3.9401 0 Td
(and)Tj
1.7291 0 Td
(custom)Tj
3.1578 0 Td
(made)Tj
-34.7128 -1.2982 Td
(concentrates.)Tj
0.83 0.64 0.02 0 k
5.5048 0 Td
(Table)Tj
2.4434 0 Td
(2)Tj
0 g
0.6917 0 Td
(gives)Tj
2.194 0 Td
(average)Tj
3.2258 0 Td
(daily)Tj
2.1487 0 Td
(intake)Tj
2.6872 0 Td
(of)Tj
1.0148 0 Td
(dry)Tj
1.576 0 Td
(matter,)Tj
3.0898 0 Td
(energy,)Tj
3.1351 0 Td
(and)Tj
1.7291 0 Td
(protein)Tj
3.1521 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(dif-)Tj
-35.4498 -1.2983 Td
(ferent)Tj
2.5738 0 Td
(treatment)Tj
4.1329 0 Td
(groups.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 247.4645 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Adipose)Tj
3.6425 0 Td
(tissue)Tj
2.5937 0 Td
(biopsy)Tj
2.9433 0 Td
(sampling)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 230.4566 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Adipose)Tj
3.5035 0 Td
(tissue)Tj
2.4605 0 Td
(biopsies)Tj
3.4299 0 Td
(were)Tj
2.126 0 Td
(collected)Tj
3.736 0 Td
(at)Tj
0.9525 0 Td
(12)Tj
1.1735 0 Td
(months)Tj
3.2825 0 Td
(of)Tj
1.0148 0 Td
(age.)Tj
1.7688 0 Td
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1.7801 0 Td
(heifers)Tj
2.88 0 Td
(were)Tj
2.126 0 Td
(given)Tj
2.3754 0 Td
(a)Tj
0.6576 0 Td
(low)Tj
1.6555 0 Td
(epidu-)Tj
-34.9226 -1.2982 Td
(ral)Tj
1.2699 0 Td
(anesthesia)Tj
4.2973 0 Td
(of)Tj
1.0148 0 Td
(35)Tj
1.6894 0 Td
(ml)Tj
1.2869 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(local)Tj
2.0806 0 Td
(anesthetic)Tj
4.2236 0 Td
(\(Lidokel-Adrenalin)Tj
7.9653 0 Td
(vet,)Tj
1.6271 0 Td
(Kela)Tj
1.9955 0 Td
(Laboratoria)Tj
4.8869 0 Td
(NV\))Tj
-34.0041 -1.2983 Td
(prior)Tj
2.2506 0 Td
(to)Tj
1.0262 0 Td
(sampling.)Tj
4.1045 0 Td
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5.7827 0 Td
(adipose)Tj
3.2598 0 Td
(tissue)Tj
2.4548 0 Td
(biopsies)Tj
3.4299 0 Td
(were)Tj
2.1259 0 Td
(harvested)Tj
4.0422 0 Td
(lateral)Tj
2.6702 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(tail)Tj
1.4684 0 Td
(base.)Tj
-35.1097 -1.3039 Td
(Each)Tj
2.1713 0 Td
(biopsy)Tj
2.8346 0 Td
(consisted)Tj
3.9401 0 Td
(of)Tj
1.0148 0 Td
(0.51.5)Tj
3.0898 0 Td
(g)Tj
0.686 0 Td
(of)Tj
1.0148 0 Td
(adipose)Tj
3.2541 0 Td
(tissue.)Tj
2.6872 0 Td
(Biopsies)Tj
3.5036 0 Td
(were)Tj
2.1203 0 Td
(immediately)Tj
5.2044 0 Td
(snap)Tj
2.0806 0 Td
(frozen)Tj
2.7723 0 Td
(in)Tj
-36.3739 -1.2983 Td
(liquid)Tj
2.5625 0 Td
(nitrogen,)Tj
3.8721 0 Td
(and)Tj
1.7347 0 Td
(subsequently)Tj
5.4028 0 Td
(stored)Tj
2.71 0 Td
(at)Tj
0.9524 0 Td
(-80C.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(RNA)Tj
2.3102 0 Td
(extraction)Tj
4.4646 0 Td
(and)Tj
1.7906 0 Td
(sequencing)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 134.9858 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Each)Tj
2.1713 0 Td
(adipose)Tj
3.2598 0 Td
(tissue)Tj
2.4605 0 Td
(biopsy)Tj
2.8289 0 Td
(was)Tj
1.7008 0 Td
(crushed)Tj
3.3845 0 Td
(on)Tj
1.2756 0 Td
(dry)Tj
1.5704 0 Td
(ice,)Tj
1.559 0 Td
(and)Tj
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BT
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1 TL
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(N8)Tj
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q
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1 J
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