Readme file for For repository.xlsx Title: Rusitec Experiment for multi-species swards Project memebrs: Ali Sultan Khan, John A. Finn, Alexandre De Menezes, Stuart Kirwan, Sinead Waters, Dominika Krol Corresponding emails: Stuart.Kirwan@teagasc.ie; stuart.kirwan.1@ucdconnect.ie ----------------------------------------- Spreadsheet contains the data for the methane emissions, pH and ruminal fermentation, volatile fatty acids, hydrogen balance and diet digestibilty related responses for a Rusitec experiment **that compared eight different forages**. Experiment was conducted between 01/12/2021 to 22/12/2021 at Teagasc Grange. Forages (and code for 'Treatment' column): High nitrogen perennial ryegrass (300NPRG) Low nitrogen perennial ryegrass (150NPRG) Timothy (TIM) Red Clover (RC) White Clover (WC) Chicory (CHI) Plantain (PL) Six-species Mix (MIX) Experimental design: 32 vessels were used with 8 vessels in each of four machines. Treatments were blocked by machine, so each machine had 1 replicate of each treatment. ----------------------------------------- The Excel spreadsheet contains - 224 observations (rows) + 1 row header - 26 variables (columns) - Column delimiter = , - Missing value = . Note: pH for overflow starts from row 66, whereas volatile fatty acids, hydrogen balance, Ammonia from vessel and overflow values start from row 98 and finish at 193 leading to 96 values for volatile fatty acids, hydrogen balance, ammonia from vessel and overflow values ----------------------------------------- Variables Format Unit Explanation Treatment Alphabet NA Diet names Machine Numeric NA Machine/block Day Numeric NA Analysis days (14-20) Vessel Numeric NA Vessel identifier (1-32) DMD Numeric Per cent Dry matter digestibility OMD Numeric Per cent Organic matter digestibility CPD Numeric Per cent Crude protein digestibility NDFD Numeric Per cent Neutral detergent fiber digestibility ADFD Numeric Per cent Acid detergent fibre digestibility pHVessel Numeric pH pH in fermentation vessels pHoverflow Numeric pH pH of effluent from the overflow jars NH3invesl Numeric milligrams per liter Ammonia concentration in fermentation vessel NH3inOvrflo Numeric milligrams per liter Ammonia concentration in overflow liquid TVFA Numeric millimoles per day Total volatile fatty acids Acetate Numeric millimoles per day Acetate production Propionate Numeric millimoles per day Propionate production Butyrate Numeric millimoles per day Butyrate production AP_Ratio Numeric ratio Acetate to propionate ratio mmolch4perday Numeric millimoles per day Amount of methane produced in moles ch4mmolpergramOMD Numeric millimoles per g of OM digested Amount of methane produced relative to a unit of organic matter digested Totalgasvolume Numeric liters per day Gas produced over 24 hours in liter per day H_produced Numeric millimoles per day Hydrogen produced H_incorporated Numeric millimoles per day Hydrogen incorporated H_recovered Numeric millimoles per day Hydrogen recovered Methane numeric percent of total gas produced Percnatge of methane in the gas prodcued over 24 hours CH4volume Numeric liters per day Volume of methane in total gas produced