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Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci
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2020-08-10
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Hill, D., O’Connor, P.M., Altermann, E. et al. Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci. Sci Rep 10, 13431 (2020). https://doi.org/10.1038/s41598-020-70328-z
Abstract
Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin
with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin
A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters
encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly
variable and appear to have undergone gene shufing with other streptococcal species. Gallocin D was
analysed in laboratory-based experiments. The mature peptides are 3,343± 1 Da and 3,019 ± 1 Da and
could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain
EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S.
gallolyticus to colonize the colon where they have been associated with colorectal cancer.