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Lactobacillus gasseri APC 678 Reduces Shedding of the Pathogen Clostridium difficile in a Murine Model
Quigley, Lisa Coakley, Mairéad Alemayehu, Debebe Rea, Mary C. Casey, Patrick G. O’Sullivan, Órla Murphy, Eileen Kiely, Barry Cotter, Paul D. Hill, Colin
Quigley, Lisa
Coakley, Mairéad
Alemayehu, Debebe
Rea, Mary C.
Casey, Patrick G.
O’Sullivan, Órla
Murphy, Eileen
Kiely, Barry
Cotter, Paul D.
Hill, Colin
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2019-02-20
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Quigley L, Coakley M, Alemayehu D, Rea MC, Casey PG, O’Sullivan Ó, Murphy E, Kiely B, Cotter PD, Hill C and Ross RP (2019) Lactobacillus gasseri APC 678 Reduces Shedding of the Pathogen Clostridium difficile in a Murine Model. Front. Microbiol. 10:273. doi: 10.3389/fmicb.2019.00273
Abstract
Clostridium difficile is a common cause of health-care acquired diarrhea, resulting in a
spectrum of disease from mild diarrhea to life-threatening illness. Sixty Lactobacillus
strains were screened for anti-C. difficile activity using a co-culture method. Based
on their ability to inhibit C. difficile, L. gasseri APC 678 and L. rhamnosus DPC 6111
were selected for study in a murine model of C. difficile infection. L. gasseri ATCC
33323, was included as a control. It was established that, relative to control mice not
fed Lactobacillus, feeding with L. gasseri APC 678 resulted in a significant reduction
by day 7 (8-fold, p = 0.017) of viable C. difficile VPI 10463 in the feces of mice. In
contrast, neither L. rhamnosus DPC 6111 nor L. gasseri ATCC 33323 significantly
reduced fecal C. difficile shedding. Sequencing of the cecal microbiota showed that
in mice fed L. gasseri APC 678 there was a significant increase in bacterial diversity
across a number of indices when compared to the control or other Lactobacillus-fed
groups. There was no significant change in the relative abundance of Firmicutes or
Bacteroidetes in the group fed L. gasseri APC 678 relative to the control, while the
groups fed L. rhamnosus DPC 6111 or L. gasseri ATCC 33323 showed a significant
decrease in the relative abundance of Firmicutes (p = 0.002 and p = 0.019, respectively)
and a significant increase in Bacteroidetes (p = 0.002 and p = 0.023, respectively).
These results highlight the potential of L. gasseri APC 678 as a live therapeutic agent to
target C. difficile infection.