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Do differences in the endometrial transcriptome between uterine horns ipsilateral and contralateral to the corpus luteum influence conceptus growth to day 14 in cattle?
Sánchez, José María Passaro, Claudia Forde, Niamh Browne, John A Behura, Susanta K Fernández-Fuertes, Beatriz Mathew, Daniel J Kelly, Alan K Butler, Stephen T Spencer, Thomas E
Sánchez, José María
Passaro, Claudia
Forde, Niamh
Browne, John A
Behura, Susanta K
Fernández-Fuertes, Beatriz
Mathew, Daniel J
Kelly, Alan K
Butler, Stephen T
Spencer, Thomas E
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2018-08-18
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José María Sánchez, Claudia Passaro, Niamh Forde, John A Browne, Susanta K Behura, Beatriz Fernández-Fuertes, Daniel J Mathew, Alan K Kelly, Stephen T Butler, Thomas E Spencer, Pat Lonergan, Do differences in the endometrial transcriptome between uterine horns ipsilateral and contralateral to the corpus luteum influence conceptus growth to day 14 in cattle?, Biology of Reproduction, Volume 100, Issue 1, January 2019, Pages 86–100, https://doi.org/10.1093/biolre/ioy185
Abstract
Embryo transfer to the uterine horn contralateral to the ovary containing the corpus luteum (CL)
negatively impacts pregnancy establishment in cattle. Our aim was to compare the transcriptome
and ability of the ipsilateral and contralateral uterine horns to support preimplantation conceptus
survival and growth to day 14. In experiment 1, endometrial samples from both horns were collected from synchronized heifers slaughtered on day 5, 7, 13, or 16 post-estrus (n = 5 per time)
and subjected to RNA sequencing. In experiment 2, 10 day 7 in vitro produced blastocysts were
transferred into the uterine horn ipsilateral (n = 9) or contralateral to the CL (n = 8) or into both
horns (i.e., bilateral, n = 9) of synchronized recipient heifers. Reproductive tracts were recovered
at slaughter on day 14, and the number and dimensions of recovered conceptuses were recorded
for each horn. A total of 217, 54, 14, and 18 differentially expressed genes (>2-fold change, FDR
P < 0.05) were detected between ipsilateral and contralateral horns on days 5, 7, 13, and 16, respectively, with signaling pathways regulating pluripotency of stem cells, ErbB signaling pathway,
and mTOR signaling pathway amongst the top canonical pathways. Site of embryo transfer did
not affect recovery rate (48.0%, 168/350) or length of conceptuses (mean ± SE 2.85 ± 0.27 mm).
Although differences in gene expression exist between the endometrium of uterine horns ipsilateral and contralateral to the CL in cattle, they do not impact conceptus survival or length between
day 7 and 14.