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The pattern of Campylobacter contamination on broiler farms; external and internal sources

Battersby, T.
; 
Whyte, P.
; 
Bolton, Declan
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Keywords
broilers, Campylobacter, broiler farms, sources, dissemination routes
Date
2016-03-07
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Food Safety
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URI
http://hdl.handle.net/11019/3607
Citation
Battersby T, Whyte P, Bolton DJ. The pattern of Campylobacter contamination on broiler farms; external and internal sources. J Appl Microbiol. 2016 Apr;120(4):1108-18. doi: 10.1111/jam.13066. Epub 2016 Mar 7. PMID: 26788933.
Abstract
Aim: The aim of this study was to apply the most sensitive molecular techniques in combination with culture-based methods to characterize broiler farms in terms of the timeline (‘appearance’ and ‘pattern’) of Campylobacter contamination prior to and post detection in the birds. Methods and Results: Faecal and environmental samples were collected from three broiler farms (two flocks per farm). Real-time PCR was used to test for the presence of Campylobacter. Culture-based methods (enrichment and direct plating) were also applied and isolates were subject to a range of confirmatory tests before speciation (multiplex PCR). All flocks were colonized by Campylobacter before first thin and a similar pattern of Campylobacter contamination was observed; (day 1) a range of external and internal samples real-time PCR positive but culture negative; (day 0) chicks negative; (6–9 days pre-detection in the birds) internal samples (feeders, drinkers, barrier and/or bird weigh) culture positive and (post broiler infection) increasing concentrations of Campylobacter in internal samples but also on the tarmac apron and anteroom. Conclusion: It was concluded that; (i) vertical transmission did not occur; (ii) the environment was a potential source of Campylobacter; (iii) testing areas frequented by all birds (e.g. feeders and drinkers), may offer an opportunity for early Campylobacter detection and (iv) once the broilers are infected with Campylobacter, these bacteria are spread from the birds, through the anteroom to the areas surrounding the broiler house, highlighting the need for improved biosecurity. Significance and Impact of the Study: This study has established the pattern of Campylobacter contamination on broiler farms, identified an early detection opportunity, highlighted the need to better understand the role of viable but nonculturable Campylobacter in the ecology of Campylobacter on broiler farms and demonstrated the need for improved biosecurity to prevent the spread of Campylobacter from within the house to the surrounding environment.
Funder
Department of Agriculture, Food and Marine (Ireland)
Grant Number
11/F/051
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