Loading...
Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4
Lozo, Jelena Mirkovic, Nemanja O'Connor, Paula M. Malesevic, Milka Miljkovic, Marija Polovic, Natalija Jovcic, Branko Cotter, Paul D. Kojic, Milan
Lozo, Jelena
Mirkovic, Nemanja
O'Connor, Paula M.
Malesevic, Milka
Miljkovic, Marija
Polovic, Natalija
Jovcic, Branko
Cotter, Paul D.
Kojic, Milan
Citations
Altmetric:
Date
2017-10-17
Collections
Files
Loading...
main article
Adobe PDF, 1.82 MB
Research Projects
Organizational Units
Journal Issue
Citation
J Lozo, N Mirkovic, P M O'Connor, M Malesevic, M Miljkovic, N Polovic, B Jovcic, P D. Cotter, M Kojic. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4. Applied and Environmental Microbiology Oct 2017, 83 (21) e01519-17; https://doi.org/10.1128/AEM.01519-17
Abstract
Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A.