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Short communication: Genotype-phenotype association analysis revealed different utilization ability of 2'-fucosyllactose in Bifidobacterium genus
He, Zhujun Yang, Bo Liu, Xiaoming Ross, R. Paul Stanton, Catherine Zhao, Jianxin Zhang, Hao Chen, Wei
He, Zhujun
Yang, Bo
Liu, Xiaoming
Ross, R. Paul
Stanton, Catherine
Zhao, Jianxin
Zhang, Hao
Chen, Wei
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2021-02-28
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Zhujun He, Bo Yang, Xiaoming Liu, R. Paul Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, Wei Chen, Short communication: Genotype-phenotype association analysis revealed different utilization ability of 2'-fucosyllactose in Bifidobacterium genus, Journal of Dairy Science, Volume 104, Issue 2, 2021, Pages 1518-1523, ISSN 0022-0302, https://doi.org/10.3168/jds.2020-19013.
Abstract
The oligosaccharide 2'-fucosyllactose (2'FL) in human breast milk selectively promotes the proliferation of bifidobacteria. One hundred fifty-one Bifidobacterium strains were evaluated for their capacity to utilize 2'FL based on the combination of phenotype and genotype association analysis. Through genotype analysis, 37 strains were predicted to have the ability to use 2'FL, including Bifidobacteriumbifidum, Bifidobacteriumbreve, Bifidobacteriumlongum ssp. longum, Bifidobacteriumlongum ssp. infantis, and Bifidobacteriumdentium, whereas Bifidobacteriumadolescentis, Bifidobacteriumanimalis, Bifidobacteriumpseudocatenulatum, and Bifidobacteriumangulatum could not use 2'FL. For in vitro utilization, there were noteworthy differences for 2'FL usage among different species, which were 100% consistent with genotype prediction. The results indicated that 2'FL utilization ability differed even within the same species, and Bifidobacterium followed the currently well-known pathway to utilize 2'FL, which could provide guidance to develop personalized prebiotics for different bifidobacteria via gene-trait matching analysis.