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Comparison of dairy phospholipid preparative extraction protocols in combination with analysis by high performance liquid chromatography coupled to a charged aerosol detector
Barry, Kate M. Dinan, Ted G. Murray, Brian A. Kelly, Philip M.
Barry, Kate M.
Dinan, Ted G.
Murray, Brian A.
Kelly, Philip M.
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2016-05-31
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Kate M. Barry, Ted G. Dinan, Brian A. Murray, Philip M. Kelly, Comparison of dairy phospholipid preparative extraction protocols in combination with analysis by high performance liquid chromatography coupled to a charged aerosol detector, International Dairy Journal, Volume 56, 2016, Pages 179-185, ISSN 0958-6946, https://doi.org/10.1016/j.idairyj.2016.01.022.
Abstract
Different fat extraction methodologies, Röse Gottlieb (RG), Folch with CaCl2 (F1) and Folch without CaCl2 (F2) were evaluated alongside a high performance liquid chromatography (HPLC)-based analytical protocol involving a charged aerosol detector (CAD) to optimise the separation and quantification of dairy phospholipids (PLs). Total PL recovery by F2 proved to be 1.8- and 2.5-fold greater than RG and F1, respectively. Alteration of the HPLC elution program maximised peak separation and reduced co-elution of individual PLs detected by CAD. Two-fold reduction in retention time variability was achieved, <0.15 min, compared with the original method. Improved PL recovery was reflected in the higher PL values obtained from different dairy streams using the optimised protocol, 35.32 ± 0.01% and 46.09 ± 0.01% total PL, for buttermilk and butter serum respectively. Phosphatidylinositol and phosphatidylserine, in particular, demonstrated increases of between 2% and 7%, of total PL, compared with previous studies.