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Biodegradable Microparticles as Non-Live Viral Vectors for Respiratory Tract Vaccination.
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2001-03-01
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Earley, B., Kavanagh, O., Adair, B., Biodegradable Microparticles as Non-Live Viral Vectors for Respiratory Tract Vaccination, End of Project Reports, Teagasc, 2001.
Abstract
The potential of a microparticulate vaccine delivery system in
eliciting a specific humoral response in the upper respiratory tract of
calves was evaluated. Microparticles composed of poly(lactideco-
glycolide) containing ovalbumin, a model immunogen, were
prepared by a solvent evaporation technique. The microparticles
were under 10μm in diameter as determined by fluorescence
activated cell sorter (FACS) analysis.
Following immunisation, the microparticles induced production of
specific secretory IgA (sIgA) in nasal samples.The sIgA was detected
after only one week and persisted throughout the length of the study.
Additionally, the effects of microencapsulated synthetic peptides
(F peptide (0.5mg) and G peptide (0.5mg), representing known
protective epitopes against bovine respiratory syncytial virus (BRSV),
on the cellular and humoral immune responses of calves were
investigated.
∗ No significant change in the cellular immune response was
detected.
* The secretory IgA response was significantly more prolonged
following administration of the SF (F111-148) peptide when
compared with the SG (G174-187) peptide.
* It is concluded that microparticles incorporating antigens show
potential in the quest for generating complete protection in the
young bovine against respiratory tract pathogens.
* Vaccination of calves with the SF (F111-148) and SG (G174-187)
peptide resulted in a significant reduction in the requirement to treat with
antibiotics for respiratory disease in the post-vaccination period.