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Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity
Russo, Daniela Kenny, Owen Smyth, Thomas J. Milella, Luigi Diop, Moussoukhoye Sissokho Rai, Dilip K. Brunton, Nigel Hossain, Mohammad Billal
Russo, Daniela
Kenny, Owen
Smyth, Thomas J.
Milella, Luigi
Diop, Moussoukhoye Sissokho
Rai, Dilip K.
Brunton, Nigel
Hossain, Mohammad Billal
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2013-04-29
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Russo, D., Kenny, O., Smyth, T., Milella, L., Hossain, M., Diop, M., Rai, D. and Brunton, N. Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity. ISRN Chromatography, 2013, 1-11. doi: http://dx.doi.org/10.1155/2013/283462
Abstract
High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to investigate the differences in phytochemicals in roots, bark, and leaf of Sclerocarya birrea (marula) for methanol and water extracts that exhibited the best antioxidant activities. As many as 36 compounds were observed in the extracts of these tissues of which 27 phenolic compounds were tentatively identified. The HPLC-MS/MS results showed flavonoid glycosides were prominent in leaf extracts while the galloylated tannins were largely in bark and root extracts. Four flavonoid glycosides that were reported for the first time in the marula leaf have been identified. The HPLC-MS/MS studies also illustrated different degrees (highest degree = 3) of oligomerisation and galloylation of tannins in the bark and root extracts.