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Using a reactionâdiffusion model to estimate day respiration and reassimilation of (photo)respiredCO2in leaves
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2019-05-11
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Berghuijs, H.N.C., Yin, X., Ho, Q.T., Retta, M.A., NicolaĂŻ, B.M. and Struik, P.C. (2019), Using a reactionâdiffusion model to estimate day respiration and reassimilation of (photo)respired CO2 in leaves. New Phytol, 223: 619-631. https://doi.org/10.1111/nph.15857
Abstract
Methods using gas exchange measurements to estimate respiration in the light (day respiration Rd) make implicit assumptions about reassimilation of (photo)respired CO2; however, this
reassimilation depends on the positions of mitochondria.
We used a reaction-diffusion model without making these assumptions to analyse
datasets on gas exchange, chlorophyll fluorescence and anatomy for tomato leaves. We
investigated how Rd values obtained by the Kok and the Yin methods are affected by
these assumptions and how those by the Laisk method are affected by the positions of
mitochondria.
The Kok method always underestimated Rd. Estimates of Rd by the Yin method and by the
reaction-diffusion model agreed only for nonphotorespiratory conditions. Both the Yin and
Kok methods ignore reassimilation of (photo)respired CO2, and thus underestimated Rd for
photorespiratory conditions, but this was less so in the Yin than in the Kok method. Estimates
by the Laisk method were affected by assumed positions of mitochondria. It did not work if
mitochondria were in the cytosol between the plasmamembrane and the chloroplast envelope. However, mitochondria were found to be most likely between the tonoplast and chloroplasts.
Our reaction-diffusion model effectively estimates Rd, enlightens the dependence of Rd
estimates on reassimilation and clarifies (dis)advantages of existing methods.