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A note on the use of FTA™ technology for storage of blood samples for DNA analysis and removal of PCR inhibitors
Mullen, Michael P. ; Howard, Dawn J. ; Powell, Richard ; Hanrahan, James P
Mullen, Michael P.
Howard, Dawn J.
Powell, Richard
Hanrahan, James P
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2009
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note on use of FTA.pdf
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M.P. Mullen, D.J. Howard, R. Powell and J.P. Hanrahan. A note on the use of FTA™ technology for storage of blood samples for DNA analysis and removal of PCR inhibitors. Irish Journal of Agricultural and Food Research 48: 109–113, 2009
Abstract
FTA™ technology is widely used across many molecular disciplines for sample
capture, storage and analysis. The use of this technology for the long-term storage
of blood samples for DNA analysis was examined as well as its potential to remove
inhibitors from DNA samples previously extracted from blood with PCR inhibitors
remaining. It was found that blood spots stored on FTA™ cards for 8 years at
room temperature gave successful PCR products and that FTA™ cards are a useful
tool for removing substances in samples which interfere with or inhibit, the PCR
reaction.
