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Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species Solanum microdontum and Solanum pampasense
Meade, Fergus Hutten, Ronald Wagener, Silke Prigge, Vanessa Dalton, Emmet Kirk, Hanne Grethe Griffin, Denis Milbourne, Dan
Meade, Fergus
Hutten, Ronald
Wagener, Silke
Prigge, Vanessa
Dalton, Emmet
Kirk, Hanne Grethe
Griffin, Denis
Milbourne, Dan
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2020-06-30
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Meade, F.; Hutten, R.; Wagener, S.; Prigge, V.; Dalton, E.; Kirk, H.G.; Griffin, D.; Milbourne, D. Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species Solanum microdontum and Solanum pampasense. Genes 2020, 11, 732. https://doi.org/10.3390/genes11070732
Abstract
Wild potato species continue to be a rich source of genes for resistance to late blight in potato
breeding. Whilst many dominant resistance genes from such sources have been characterised and
used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the
resistance can be identified and tagged using molecular markers. In this study, F1 populations were
created from crosses between blight susceptible parents and lines exhibiting strong partial resistance
to late blight derived from the South American wild species Solanum microdontum and Solanum
pampasense. Both populations exhibited continuous variation for resistance to late blight over multiple
field-testing seasons. High density genetic maps were created using single nucleotide polymorphism
(SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were
consistently expressed over multiple years in both populations. In the population created with the
S. microdontum source, QTLs for resistance consistently expressed over three years and explaining a
large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further
resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016
only. In the population created with the S. pampasense source, QTLs for resistance were found in over
two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span
known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic
models following the effect of the presence or absence of SNPs associated with consistently effective
loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress
and pyramid these loci have potential in resistance breeding strategies.