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Association of genetic polymorphisms related to Johne’s disease with estimated breeding values of Holstein sires for milk ELISA test scores
Mallikarjunappa, Sanjay Schenkel, Flavio S. Brito, Luiz F. Bissonnette, Nathalie Miglior, Filippo Chesnais, Jacques Lohuis, Michae Meade, Kieran G. Karrow, Niel A
Mallikarjunappa, Sanjay
Schenkel, Flavio S.
Brito, Luiz F.
Bissonnette, Nathalie
Miglior, Filippo
Chesnais, Jacques
Lohuis, Michae
Meade, Kieran G.
Karrow, Niel A
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2020-05-27
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Mallikarjunappa, S., Schenkel, F.S., Brito, L.F. et al. Association of genetic polymorphisms related to Johne’s disease with estimated breeding values of Holstein sires for milk ELISA test scores. BMC Vet Res 16, 165 (2020). https://doi.org/10.1186/s12917-020-02381-9
Abstract
Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium
subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment
options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status
based on published genome-wide association and candidate gene studies. The main objective of this study was to
validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’
estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and
26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR <
0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified
four SNPs that were associated with sire EBVs (FDR < 0.05). This includes two SNPs that were common with GQLS
(rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and
BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight
the need to further investigate the genomic regions harboring these SNPs.