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The in vitro host cell immune response to bovine-adapted Staphylococcus aureus varies according to bacterial lineage
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2019-04-16
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Murphy, M.P., Niedziela, D.A., Leonard, F.C. et al. The in vitro host cell immune response to bovine-adapted Staphylococcus aureus varies according to bacterial lineage. Sci Rep 9, 6134 (2019). https://doi.org/10.1038/s41598-019-42424-2
Abstract
Mastitis is the most economically important disease afecting dairy cattle worldwide. Staphylococcus
aureus is a highly prevalent cause of mastitis, causing infections ranging from sub-clinical to
gangrenous. However, the interaction between the genotype of the infecting strain of S. aureus and
the host response remains largely uncharacterised. To better understand the variation in presentation
and outcomes of S. aureus-mediated bovine mastitis, we studied the interaction of a panel of mastitis
isolates from several prominent bovine-associated lineages with bovine mammary epithelial cells
(bMEC) and neutrophils. Signifcant diferences in immune gene expression by infected primary or
immortalised bMEC, or their elaboration of neutrophil chemoattractants, were observed and were
dependent on the lineage of the infecting strain. Diferences were also apparent in the invasiveness
of S. aureus strains and their ability to survive killing by neutrophils. Our results demonstrate that a
range of immune responses occur, suggesting the importance of S. aureus strain in dictating mastitis
disease course. S. aureus lineages may therefore have adopted difering strategies for exploitation of
the intramammary niche. Consequently, improved diagnosis of infecting lineage may enable better
prognosis for S. aureus mastitis and reduce morbidity and economic loss.