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Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production

Yang, Bo
; 
Chen, Haiqin
; 
Stanton, Catherine
; 
Chen, Yong Q.
; 
Zhang, Hao
; 
Chen, Wei
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Keywords
bifidobacteria, bioconversion, conjugated linolenic acid, isolation
Date
2016-09-22
Collections
Food Biosciences
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URI
http://hdl.handle.net/11019/2897
Citation
Bo Yang, Haiqin Chen, Catherine Stanton, Yong Q. Chen, Hao Zhang & Wei Chen (2017) Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production, Bioengineered, 8:3, 232-238, DOI: 10.1080/21655979.2016.1222996
Abstract
Conjugated linolenic acid (CLNA) is a family of isomers of linolenic acid with a number of healthassociated benefits, which has been attracting great interest. Microbial CLNA producers are potentially an alternative source of CLNA for human nutrition. In present study, 16 neonate feces were collected and used for Bifidobacteria isolation, from which 25 bifidobacteria isolates were obtained. The bifidobacteria isolates were identified using 16s rDNA sequencing as Bifidobacterium adolescentis, B. breve, B. longum and B. pseudocatenulatum. These isolates were further investigated for their ability to produce CLNA using linolenic acid as substrate via GC-MS. The results showed most of the isolates could convert free linolenic acid into c9,t11,c15-CLNA and t9,t11,c15-CLNA at different levels. B. pseudocatenulatum was the most effective CLNA producer, which converted 86.91% of linolenic acid to c9,t11,c15-CLNA and 3.59% of to t9,t11,c15-CLNA isomer and the isolate exhibited to accumulate CLNA during 72 h culturing in which most CLNA isomers were in the supernatant fluid. The results indicated that utilization of this isolate for CLNA production will eliminate the purification process.
Funder
National Natural Science Foundation of China
the Program for New Century Excellent Talents
the Program for Changjiang Scholars and Innovative Research Team in University
the National Natural Science Foundation of Jiangsu Province
the Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control.”
Grant Number
Nos. 31571810, 31530056
NCET-13-0831
IRT1249
BK20150141
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