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Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
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2019-11-28
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Lanigan, N., Kelly, E., Arzamasov, A.A. et al. Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators. Sci Rep 9, 17851 (2019). https://doi.org/10.1038/s41598-019-54229-4
Abstract
Bifdobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing
environmental conditions, which require rapid adjustments in gene expression. Here, we show that two
predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating
the central, carbohydrate-associated metabolic pathway (the so-called phosphoketolase pathway
or bifd shunt) of the gut commensal Bifdobacterium breve UCC2003. These TFs appear to not only
control transcription of genes involved in the bifd shunt and each other, but also seem to commonly
and directly afect transcription of other TF-encoding genes, as well as genes related to uptake and
metabolism of various carbohydrates. This complex and interactive network of AraQ/MalR1-mediated
gene regulation provides previously unknown insights into the governance of carbon metabolism in
bifdobacteria.