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In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
Egan, Kevin Field, Des Ross, R. Paul Cotter, Paul Hill, Colin
Egan, Kevin
Field, Des
Ross, R. Paul
Cotter, Paul
Hill, Colin
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2018-09-20
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Egan K, Field D, Ross RP, Cotter PD and Hill C (2018) In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus. Front. Microbiol. 9:2116. doi: 10.3389/fmicb.2018.02116
Abstract
The thermophilic, endospore-forming genus of Geobacillus has historically been
associated with spoilage of canned food. However, in recent years it has become the
subject of much attention due its biotechnological potential in areas such as enzyme and
biofuel applications. One aspect of this genus that has not been fully explored or realized
is its use as a source of novel forms of the ribosomally synthesized antimicrobial peptides
known as bacteriocins. To date only two bacteriocins have been fully characterized
within this genus, i.e., Geobacillin I and II, with only a small number of others partially
characterized. Here we bioinformatically investigate the potential of this genus as a
source of novel bacteriocins through the use of the in silico screening software BAGEL3,
which scans publically available genomes for potential bacteriocin gene clusters. In
this study we examined the association of bacteriocin gene presence with niche and
phylogenetic position within the genus. We also identified a number of candidates from
multiple bacteriocin classes which may be promising antimicrobial candidates when
investigated in vitro in future studies.