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A Major Facilitator Superfamily Peptide Transporter From Fusarium oxysporum Influences Bioethanol Production From Lignocellulosic Material
Nugent, Brian Ali, Shahin S Mullins, Ewen Doohan, Fiona M.
Nugent, Brian
Ali, Shahin S
Mullins, Ewen
Doohan, Fiona M.
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2019-02-26
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Nugent B, Ali SS, Mullins E and Doohan FM (2019) A Major Facilitator Superfamily Peptide Transporter From Fusarium oxysporum Influences Bioethanol Production From Lignocellulosic Material. Front. Microbiol. 10:295. doi: 10.3389/fmicb.2019.00295
Abstract
Fusarium oxysporum is a leading microbial agent in the emerging consolidated
bioprocessing (CBP) industry owing to its capability to infiltrate the plant’s lignin barrier
and degrade complex carbohydrates to value-added chemicals such as bioethanol in
a single step. Membrane transport of nutrients is a key factor in successful microbial
colonization of host tissue. This study assessed the impact of a peptide transporter on
F. oxysporum’s ability to convert lignocellulosic straw to ethanol. We characterized a
novel F. oxysporum peptide transporter (FoPTR2) of the dipeptide/tripeptide transporter
(PTR) class. FoPTR2 represents a novel transporter with high homology to the
Trichoderma sp. peptide transporters ThPTR2 and TrEST-AO793. Its expression level
was highly activated in nitrogen-poor environments, which is a characteristic of PTR
class peptide transporters. Overexpression and post-translational gene silencing of the
FoPTR2 in F. oxysporum affected the peptide transport capacity and ethanol yielded
from a both a wheat straw/bran mix and glucose. Thus, we conclude that it FoPTR2
plays a role in the nutrient acquisition system of F. oxysporum which serves to not only
enhance fungal fitness but also CBP efficacy.