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Application of Flow Cytometry to the Detection of Pathogenic Bacteria
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2015
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Kennedy, D. and Wilkinson, M.G. (2015) “Application of flow cytometry to the detection of pathogenic bacteria,” Flow Cytometry in Microbiology: Technology and Applications, pp. 91–108. Available at: https://doi.org/10.21775/9781910190111.05.
Abstract
Outbreaks of infections have emphasized the
necessity for rapid and economic detection methods
for pathogens in samples ranging from those of
clinical origin to food products during production and
retail storage, and increasingly, in environmental
samples. Flow cytometry (FCM) allows the rapid
acquisition of multi-parametric data regarding cell
populations within fluidised samples. However, the
application of FCM to pathogen detection depends
on the availability of specific fluorescent probes
such as antibodies and RNA probes capable of
detecting and isolating pathogens from these
diverse samples. A particular issue for FCM
methodology is the ability to recover and
discriminate bacteria from the sample matrix which
may pose a major technical hurdle towards accurate
and sensitive analysis. This review article focuses
on detection of pathogens using FCM in samples
originating from food, water, environmental and
clinical sources and outlines the current state of the
art and potential future applications.