Iodine tends to be supplemented at farm level in the expectation of increasing cow health and fertility. There is concern that such practices may result in high milk iodine, which could affect ingredients for infant formula and, thus, dairy export markets. The objective of this study was to quantify the effect of iodine fortified feed and teat disinfection practices of dairy cows on milk iodine concentration. Thirty lactating cows were fed 7 kg, 3 kg (10 mg iodine/kg) and 0 kg of concentrate feed during 3 periods of 35 days each. During the first 14 days of each period, cows were on dietary iodine treatments only; during days 15–21, one of three teat disinfection treatments (n = 10) was applied (in addition to the dietary iodine treatments): non-iodine (chlorhexidine) post-milking spray; 0.5% iodine spray post-milking; 0.5% iodine spray pre- and post-milking. Cow milk yield was 21.3 kg/day. Individual cow milk samples were analysed for iodine concentration on 2 days at the end of each treatment period. Dietary supplementation of iodine at both 30 mg and 70 mg/day, when compared to the diet with no supplement, increased milk iodine concentrations significantly (P < 0.001) from 449 to 1034 and 915 μg/kg, respectively. Teat disinfection both pre- and post-milking increased milk iodine concentration at each of the dietary supplementation levels of 0, 30 and 70 mg/day compared with a non-iodine teat disinfectant (P < 0.001). In conclusion, both dietary iodine supplementation and teat disinfection iodine increased milk iodine concentrations in an additive manner, exceeding common target values of 250 μg/kg. As both iodine treatments can occur simultaneously on farm, supplementation strategies should be monitored.