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dc.contributor.authorMoran, Mary G.*
dc.contributor.authorDix, Philip J.*
dc.contributor.authorBurke, James I.*
dc.date.accessioned2016-08-10T11:57:21Z
dc.date.available2016-08-10T11:57:21Z
dc.date.issued2016-01-13
dc.identifier.citationMoran, M., Dix, P. & Burke, J. (2016). Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS). Irish Journal of Agricultural and Food Research, 54(1), pp. 48-58. doi:10.1515/ijafr-2015-0005en_GB
dc.identifier.issn2009-9029
dc.identifier.issn0791-6833 (print)
dc.identifier.urihttp://hdl.handle.net/11019/1042 http://dx.doi.org/10.1515/ijafr-2015-0005
dc.identifier.urihttp://dx.doi.org/10.1515/ijafr-2015-0005
dc.descriptionpeer-revieweden_GB
dc.description.abstractPhysiological leaf spot (PLS) is a disorder of spring barley (Hordeum vulgare L.), which has become more pronounced in recent years. The initial symptoms are small chlorotic/brown spots on the upper four leaves, which may develop into necrotic lesions with an irregular shape. As PLS occurs on leaves that are directly exposed to sunlight, it is thought that high light stress could be a trigger for the condition. This study concentrates on two cultivars, Cooper and Crusader, which display differential sensitivity to PLS. Biochemical measurements and enzyme assays revealed substantial difference in levels of ascorbate, type III peroxidases, and superoxide dismutase between the chosen cultivars during the 2003 growing season. A global gene expression study, using these field samples, was performed by microarray analysis. This supported the biochemical findings and highlighted additional sets of genes differentially expressed between the cultivars. Transcripts of particular interest, which appeared, included calcium signalling genes, cold-responsive genes and those involved in the assembly of Photosystem I. We conclude that susceptibility to PLS is related to levels of expression of genes with a role in countering the effects of oxidative stress.en_GB
dc.description.sponsorshipTeagasc Walsh Fellowship Programmeen_GB
dc.language.isoenen_GB
dc.publisherTeagasc (Agriculture and Food Development Authority), Irelanden_GB
dc.relation.ispartofseriesIrish Journal of Agricultural and Food Research;
dc.subjectAbiotic stressen_GB
dc.subjectNon-parasitic necrosisen_GB
dc.subjectPhysiological leaf spoten_GB
dc.titleMicroarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS)en_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T08:29:26Z


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