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dc.contributor.authorLeong, Dara*
dc.contributor.authorAlvarez-Ordonez, Avelino*
dc.contributor.authorJordan, Kieran*
dc.date.accessioned2016-08-12T14:14:32Z
dc.date.available2016-08-12T14:14:32Z
dc.date.issued30/12/2015
dc.identifier.citationLeong, D., Alvarez-Ordóñez, A. & Jordan, K. (2015). A note on challenge trials to determine the growth of Listeria monocytogenes on mushrooms (Agaricus bisporus). Irish Journal of Agricultural and Food Research, 54(2), pp. 121-125. doi:10.1515/ijafr-2015-0013en_GB
dc.identifier.issn2009-9029
dc.identifier.urihttp://hdl.handle.net/11019/1055
dc.descriptionpeer-revieweden_GB
dc.description.abstractIn the EU, food is considered safe with regard to Listeria monocytogenes if the number of micro-organisms does not exceed 100 colony forming units (cfu)/g throughout its shelf-life. Therefore, it is important to determine if a food supports growth of L. monocytogenes. Guidelines for conducting challenge tests for growth assessment of L. monocytogenes on foods were published by the European Union Reference Laboratory (EURL) in 2014. The aim of this study was to use these guidelines to determine if refrigerated, fresh, whole, closed-cap, prepackaged mushrooms (Agaricus bisporus) support the growth of L. monocytogenes. Three batches of mushrooms were artificially inoculated at approximately 100 cfu/g with a three-strain mix of L. monocytogenes and incubated for 2 days at 8°C followed by 4 days at 12°C. L. monocytogenes numbers were determined (in triplicate for each batch) on days 0, 2 and 6. Water activity, pH and total bacterial counts were also determined. There was no increase in the number of L. monocytogenes above the threshold of 0.5 log cfu/g in any of the replicates. In 8 of 9 replicates, the numbers decreased indicating that A. bisporus do not support the growth of L. monocytogenes. As the EU regulations allow < 100 cfu/g if the food cannot support growth of L. monocytogenes, the significance of this study is that mushrooms with < 100 cfu/g may be within the regulations and therefore, quantitative rather than qualitative determination may be required.en_GB
dc.description.sponsorshipSafefooden_GB
dc.language.isoenen_GB
dc.publisherTeagasc (Agriculture and Food Development Authority), Irelanden_GB
dc.relation.ispartofseriesIrish Journal of Agricultural and Food Research;vol 54
dc.subjectListeria monocytogenesen_GB
dc.subjectMushroomsen_GB
dc.subjectAgaricus bisporusen_GB
dc.subjectGrowthen_GB
dc.subjectchallenge studyen_GB
dc.subjectFood safetyen_GB
dc.titleA note on challenge trials to determine the growth of Listeria monocytogenes on mushrooms (Agaricus bisporus)en_GB
dc.typeArticleen_GB
dc.identifier.rmisMDSA-0101-6339
dc.identifier.doihttp://dx.doi.org/10.1515/ijafr-2015-0013
dc.contributor.sponsorSafefood
refterms.dateFOA2018-01-12T08:34:50Z


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