Show simple item record

dc.contributor.authorField, Des*
dc.contributor.authorO'Connor, Rory*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorHill, Colin*
dc.date.accessioned2016-08-15T15:30:31Z
dc.date.available2016-08-15T15:30:31Z
dc.date.issued18/04/2016
dc.identifier.citationField D, O’ Connor R, Cotter PD, Ross RP and Hill C (2016) In Vitro Activities of Nisin and Nisin Derivatives Alone and In Combination with Antibiotics against Staphylococcus Biofilms. Front. Microbiol. 7:508. doi: 10.3389/fmicb.2016.00508en_GB
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11019/1062
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe development and spread of pathogenic bacteria that are resistant to the existing catalog of antibiotics is a major public health threat. Biofilms are complex, sessile communities of bacteria embedded in an organic polymer matrix which serve to further enhance antimicrobial resistance. Consequently, novel compounds and innovative methods are urgently required to arrest the proliferation of drug-resistant infections in both nosocomial and community environments. Accordingly, it has been suggested that antimicrobial peptides could be used as novel natural inhibitors that can be used in formulations with synergistically acting antibiotics. Nisin is a member of the lantibiotic family of antimicrobial peptides that exhibit potent antibacterial activity against many Gram-positive bacteria. Recently we have used bioengineering strategies to enhance the activity of nisin against several high profile targets, including multi-drug resistant clinical pathogens such as methicillin-resistant Staphylococcus aureus, vancomycinresistant enterococci, staphylococci, and streptococci associated with bovine mastitis. We have also identified nisin derivatives with an enhanced ability to impair biofilm formation and to reduce the density of established biofilms of methicillin resistant S. pseudintermedius. The present study was aimed at evaluating the potential of nisin and nisin derivatives to increase the efficacy of conventional antibiotics and to assess the possibility of killing and/or eradicating biofilm-associated cells of a variety of staphylococcal targets. Growth curve-based comparisons established that combinations of derivatives nisin V C penicillin or nisin I4V C chloramphenicol had an enhanced inhibitory effect against S. aureus SA113 and S. pseudintermedius DSM21284, respectively, compared to the equivalent nisin A C antibiotic combinations or when each antimicrobial was administered alone. Furthermore, the metabolic activity of established biofilms treated with nisin V C chloramphenicol and nisin I4V C chloramphenicol combinations revealed a significant decrease in S. aureus SA113 and S. pseudintermedius DSM21284 biofilm viability, respectively, compared to the nisin A C antibiotic combinations as determined by the rapid colorimetric XTT assay. The results indicate that the activities of the nisin derivative and antibiotic combinations represent a significant improvement over that of the wild-type nisin and antibiotic combination and merit further investigation with a view to their use as anti-biofilm agents.en_GB
dc.description.sponsorshipDF,CH,PC,RR are supported by the Irish Government under the National Development Plan, through a Science Foundation Ireland (SFI)Technology and Innovation Development Award (TIDA14/TIDA/2286)to DF,a SFI Investigator awards to CH and RR(10/IN.1/B3027),SFI-PI funding(11/PI/1137)to PC and the Alimentary Pharmabiotic Centre under Grant Number SFI/12/RC/2273.
dc.language.isoenen_GB
dc.publisherFrontiers Media S. A.en_GB
dc.relation.ispartofseriesFrontiers in Microbiology;vol 7
dc.subjectBiofilmen_GB
dc.subjectbacterial resistanceen_GB
dc.subjectantimicrobial peptideen_GB
dc.subjectNisinen_GB
dc.subjectlantibioticen_GB
dc.subjectbacteriocinen_GB
dc.subjectstaphylococcien_GB
dc.subjectAntibioticsen_GB
dc.titleIn Vitro Activities of Nisin and Nisin Derivatives Alone and In Combination with Antibiotics against Staphylococcus Biofilmsen_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.3389/fmicb.2016.00508
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberTIDA14/TIDA/2286)
dc.contributor.sponsorGrantNumber10/IN.1/B3027
dc.contributor.sponsorGrantNumber11/PI/1137
dc.contributor.sponsorGrantNumberSFI/12/RC/2273
refterms.dateFOA2018-01-12T08:20:25Z


Files in this item

Thumbnail
Name:
fmicb-07-00508.pdf
Size:
3.083Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record