Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae spp. dysgalactiae in mammary epithelial cells
dc.contributor.author | O'Halloran, Fiona | * |
dc.contributor.author | Beecher, Christine | * |
dc.contributor.author | Chaurin, Valerie | * |
dc.contributor.author | Sweeney, Torres | * |
dc.contributor.author | Giblin, Linda | * |
dc.date.accessioned | 2016-09-13T15:40:08Z | |
dc.date.available | 2017-04-01T16:00:05Z | |
dc.date.issued | 24/03/2016 | |
dc.identifier.citation | Fiona O’Halloran, Christine Beecher, Valerie Chaurin, Torres Sweeney, Linda Giblin, Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae ssp. dysgalactiae in mammary epithelial cells, Journal of Dairy Science, 2016, 99(6), 4619-4628. DOI: 10.3168/jds.2015-10465. | en_GB |
dc.identifier.issn | 0022-0302 | |
dc.identifier.uri | http://hdl.handle.net/11019/1068 | |
dc.description | peer-reviewed | en_GB |
dc.description.abstract | Streptococcus dysgalactiae ssp. dysgalactiae is an important causative agent of bovine mastitis worldwide. Lactoferrin is an innate immune protein that is associated with many functions including immunomodulatory, antiproliferative, and antimicrobial properties. This study aimed to investigate the interactions between lactoferrin and a clinical bovine mastitis isolate, Strep. dysgalactiae ssp. dysgalactiae DPC5345. Initially a deliberate in vivo bovine intramammary challenge was performed with Strep. dysgalactiae DPC5345. Results demonstrated a significant difference in lactoferrin mRNA levels in milk cells between the control and infused quarters 7 h postinfusion. Milk lactoferrin levels in the Strep. dysgalactiae DPC5345 infused quarters were significantly increased compared with control quarters at 48 h postinfusion. In vitro studies demonstrated that lactoferrin had a bacteriostatic effect on the growth of Strep. dysgalactiae DPC5345 and significantly decreased the ability of the bacteria to internalize into HC-11 mammary epithelial cells. Confocal microscopy images of HC-11 cells exposed to Strep. dysgalactiae and lactoferrin further supported this effect by demonstrating reduced invasion of bacteria to HC-11 cells. The combined data suggest that a bovine immune response to Strep. dysgalactiae infection includes a significant increase in lactoferrin expression in vivo, and based on in vitro data, lactoferrin limits mammary cell invasion of this pathogen by binding to the bacteria and preventing its adherence. | en_GB |
dc.description.sponsorship | This research was funded by the Food Institutional Research Measure (Department of Agriculture, Food and Fisheries, Ireland) Projects 06RDTMFRC437 and 06RDTMFRC445, the Irish Dairy Research Trust, and the Teagasc Retooling Program under the National Development Plan. Christine Beecher was in receipt of a Teagasc Walsh Fellowship. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.ispartofseries | Journal of Dairy Science;vol 99 | |
dc.subject | Lactoferrin | en_GB |
dc.subject | Streptococcus dysgalactiae ssp. dysgalactiae | en_GB |
dc.subject | Mastitis | en_GB |
dc.title | Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae spp. dysgalactiae in mammary epithelial cells | en_GB |
dc.type | Article | en_GB |
dc.embargo.terms | 24/03/2017 | en_GB |
dc.identifier.rmis | MDDB-0208-5582 | |
dc.identifier.doi | http://dx.doi.org/10.3168/jds.2015-10465 | |
dc.contributor.sponsor | Department of Agriculture, Food and the Marine | |
dc.contributor.sponsor | Irish Dairy Levy Research Trust | |
dc.contributor.sponsor | Teagasc Walsh Fellowship Programme | |
dc.contributor.sponsorGrantNumber | 06RDTMFRC437 | |
dc.contributor.sponsorGrantNumber | 06RDTMFRC445 | |
refterms.dateFOA | 2018-01-12T08:28:00Z |
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