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    Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

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    Author
    O'Shea, Emma
    Waters, Sinead M.
    Keogh, Kate cc
    Kelly, Alan K
    Kenny, David A.
    Keyword
    Beef cattle
    Compensatory growth
    Feed efficiency
    Nutrient restriction
    Rumen epithelium
    Date
    2016-09-15
    
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    URI
    http://hdl.handle.net/11019/1077
    Citation
    O'Shea, E. et al.Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle. Journal of Animal Science and Biotechnology. 2016 Sep 15;7(1):53. DOI:10.1186/s40104-016-0114-8
    Abstract
    Background The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. Sixty Holstein Friesian bulls (mean liveweight 370 ± 35 kg; mean age 479 ± 15 d) were assigned to one of two groups: (i) restricted feed allowance (RES; n = 30) for 125 d (Period 1) followed by ad libitum access to feed for 55 d (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n = 30). Target growth rate for RES was 0.6 kg/d during Period 1. At the end of each dietary period, 15 animals from each treatment group were slaughtered and ruminal epithelial tissue and liquid digesta harvested from the ventral sac of the rumen. Real-time qPCR was used to quantify mRNA transcripts of 26 genes associated with ruminal epithelial function. Volatile fatty acid analysis of rumen fluid from individual animals was conducted using gas chromatography. Results Diet × period interactions were evident for genes involved in ketogenesis (BDH2, P = 0.017), pyruvate metabolism (LDHa, P = 0.048; PDHA1, P = 0.015) and cellular transport and structure (DSG1, P = 0.019; CACT, P = 0.027). Ruminal concentrations of propionic acid (P = 0.018) and n-valeric acid (P = 0.029) were lower in RES animals, compared with ADLIB, throughout the experiment. There was also a strong tendency (P = 0.064) toward a diet × period interaction for n-butyric with higher concentrations in RES animals, compared with ADLIB, during Period 1. Conclusions These data suggest that following nutrient restriction, the structural integrity of the rumen wall is compromised and there is upregulation of genes involved in the production of ketone bodies and breakdown of pyruvate for cellular energy. These results provide an insight into the potential molecular mechanisms regulating ruminal epithelial absorptive metabolism and growth following nutrient restriction and subsequent compensatory growth.
    Funder
    Science Foundation Ireland
    Grant Number
    09/RFP/GEN2447
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1186/s40104-016-0114-8
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    Teagasc publications in Biomed Central
    Animal & Bioscience

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