Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy
Author
Dalmasso, MarionStrain, Ronan
Neve, Horst
Franz, C.M.A.P.
Cousin, Fabien J.
Ross, R Paul
Hill, Colin
Keyword
BacteriophagesEscherichia coli
Bacterial biofilms
Viral genomics
Antibiotics
Comparative genomics
Date
09/06/2016
Metadata
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Dalmasso M, Strain R, Neve H, Franz CMAP, Cousin FJ, et al. (2016) Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy. PLoS ONE 11(6): e0156773. doi: 10.1371/journal.pone.0156773Abstract
With the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, ɸAPCEc01, ɸAPCEc02 and ɸAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10−3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.Funder
Science Foundation IrelandGrant Number
SFI/12/RC/2273ae974a485f413a2113503eed53cd6c53
http://dx.doi.org/10.1371/journal.pone.0156773