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dc.contributor.authorDalmasso, Marion
dc.contributor.authorStrain, Ronan
dc.contributor.authorNeve, Horst
dc.contributor.authorFranz, Charles M. A. P.
dc.contributor.authorCousin, Fabian J.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorHill, Colin
dc.date.accessioned2016-11-24T17:02:20Z
dc.date.available2016-11-24T17:02:20Z
dc.date.issued2016-06-09
dc.identifier.citationDalmasso M, Strain R, Neve H, Franz CMAP, Cousin FJ, et al. (2016) Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy. PLoS ONE 11(6): e0156773. doi: 10.1371/journal.pone.0156773en_GB
dc.identifier.urihttp://hdl.handle.net/11019/1087
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0156773
dc.descriptionpeer-revieweden_GB
dc.description.abstractWith the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, ɸAPCEc01, ɸAPCEc02 and ɸAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10−3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.en_GB
dc.description.sponsorshipScience Foundation Ireland r Grant Number SFI/12/RC/2273.en_GB
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLoS ONE;vol 11
dc.subjectBacteriophagesen_GB
dc.subjectEscherichia colien_GB
dc.subjectBacterial biofilmsen_GB
dc.subjectViral genomicsen_GB
dc.subjectAntibioticsen_GB
dc.subjectComparative genomicsen_GB
dc.titleThree New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapyen_GB
dc.typeArticleen_GB
refterms.dateFOA2018-01-12T08:36:53Z


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