Early Gut Microbiota Perturbations Following Intrapartum Antibiotic Prophylaxis to Prevent Group B Streptococcal Disease
Author
Mazzola, GiuseppeMurphy, Kiera
Ross, R Paul
Di Gioia, Diana
Biavati, Bruno
Corvaglia, Luigi T.
Faldella, Giacomo
STANTON, CATHERINE

Keyword
BifidobacteriumInfants
Microbiome
Antibiotic
Enterobacteriaceae
Dietary factors
bacterial diversity
Date
22/06/2016
Metadata
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Mazzola G, Murphy K, Ross RP, Di Gioia D, Biavati B, et al. (2016) Early Gut Microbiota Perturbations Following Intrapartum Antibiotic Prophylaxis to Prevent Group B Streptococcal Disease. PLoS ONE 11(6): e0157527. doi: 10.1371/journal.pone.0157527Abstract
The faecal microbiota composition of infants born to mothers receiving intrapartum antibiotic prophylaxis with ampicillin against group B Streptococcus was compared with that of control infants, at day 7 and 30 of life. Recruited newborns were both exclusive breastfed and mixed fed, in order to also study the effect of dietary factors on the microbiota composition. Massive parallel sequencing of the V3-V4 region of the 16S rRNA gene and qPCR analysis were performed. Antibiotic prophylaxis caused the most marked changes on the microbiota in breastfed infants, mainly resulting in a higher relative abundance of Enterobacteriaceae, compared with control infants (52% vs. 14%, p = 0.044) and mixed-fed infants (52% vs. 16%, p = 0.13 NS) at day 7 and in a lower bacterial diversity compared to mixed-fed infants and controls. Bifidobacteria were also particularly vulnerable and abundances were reduced in breastfed (p = 0.001) and mixed-fed antibiotic treated groups compared to non-treated groups. Reductions in bifidobacteria in antibiotic treated infants were also confirmed by qPCR. By day 30, the bifidobacterial population recovered and abundances significantly increased in both breastfed (p = 0.025) and mixed-fed (p = 0.013) antibiotic treated groups, whereas Enterobacteriaceae abundances remained highest in the breastfed antibiotic treated group (44%), compared with control infants (16%) and mixed-fed antibiotic treated group (28%). This study has therefore demonstrated the short term consequences of maternal intrapartum antibiotic prophylaxis on the infant faecal microbial population, particularly in that of breastfed infants.Funder
Department of Agriculture, Food and the Marine; European Union; Science Foundation Ireland; Global Grant Spinner project 2013; Teagasc Walsh Fellowship ProgrammeGrant Number
INFANTMET 10FDairy; MyNewGut 613979ae974a485f413a2113503eed53cd6c53
http://dx.doi.org/10.1371/journal.pone.0157527